CAS 100645-45-2

P-nitrophenyl B-D-galactofuranoside

Description:

P-nitrophenyl β-D-galactofuranoside is a synthetic compound commonly used as a substrate in biochemical assays, particularly for the detection of galactosidase enzymes. It features a p-nitrophenyl group, which serves as a chromogenic indicator, allowing for the measurement of enzymatic activity through color change upon hydrolysis. The β-D-galactofuranoside moiety is a sugar derivative, specifically a furanose form of galactose, which is linked to the p-nitrophenyl group via a glycosidic bond. This compound is typically a white to pale yellow solid and is soluble in water and organic solvents, making it versatile for laboratory applications. Its hydrolysis by galactosidases releases p-nitrophenol, which can be quantified spectrophotometrically, providing a reliable method for enzyme activity assessment. Additionally, P-nitrophenyl β-D-galactofuranoside is used in various research fields, including microbiology and biochemistry, to study enzyme kinetics and carbohydrate metabolism. Proper handling and storage are essential due to its potential reactivity and the need for stability in experimental conditions.

Formula: C₁₂H₁₅NO₈

InChI: InChI=1/C12H15NO8/c14-5-8(15)11-9(16)10(17)12(21-11)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9-,10-,11+,12-/m1/s1

Synonyms:

• 4-Nitrophenylgalactofuranoside
• 4-Np-Gf
• 4-Nitrophenyl-beta-D-galactofuranoside

4-Nitrophenyl β-D-Galactofuranoside

CAS: 100645-45-2

Formula: C₁₂H₁₅NO₈

Color and Shape: Solid

Molecular weight: 301.2494

Ref: IN-DA0002K4

4-Nitrophenyl β-D-Galactofuranoside

Controlled Product

CAS: 100645-45-2

Applications Galactofuranose is a widespread component of cell wall polysaccharides in bacteria, protozoa and fungi, but is totally absent in mammals. pNP-β-D-Galf is a convenient substrate for β-Galactofuranosidase.
References Notermans, S., et al.: Mol. Immunol., 25, 975 (1988), Henrissat, B., et al.: Biochem. J., 280, 309 (1991), Pathak, A., et al.: Bioorg. Med. Chem., 9, 3129 (2001),

Formula: C₁₂H₁₅NO₈

Color and Shape: Neat

Molecular weight: 301.25

Ref: TR-N503570

4-Nitrophenyl β-D-galactofuranoside

CAS: 100645-45-2

Chromogenic substrate for beta-galactofuranosidase

Formula: C₁₂H₁₅NO₈

Purity: Min. 98 Area-%

Color and Shape: White Powder

Molecular weight: 301.25 g/mol

Ref: 3D-EN06054