CAS 104497-79-2: 2,3-BIS(4-METHOXYPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE
CAS 104497-79-2: 2,3-BIS(4-METHOXYPHENYL)-5-PHENYLTETRAZOLIUM CHLORIDE
Description:2,3-Bis(4-methoxyphenyl)-5-phenyltetrazolium chloride, commonly referred to as MTT, is a chemical compound that belongs to the tetrazolium family. It is characterized by its vibrant colorimetric properties, which make it useful in various biological assays, particularly in cell viability and proliferation studies. The compound is soluble in water and organic solvents, allowing for versatile applications in laboratory settings. Upon reduction by viable cells, MTT is converted into a formazan product, which exhibits a deep purple color, enabling quantification through spectrophotometry. This transformation is indicative of metabolic activity, making MTT a valuable tool in cytotoxicity assays. Additionally, the presence of methoxy groups enhances its lipophilicity, influencing its interaction with biological membranes. MTT is generally considered safe for laboratory use, but standard safety precautions should be observed due to its chemical nature. Overall, its unique properties and ease of use have established MTT as a standard reagent in biochemical research.
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