CAS 1158-17-4

o-Nitrophenyl β-D-thiogalactoside

Description:

o-Nitrophenyl β-D-thiogalactoside (commonly abbreviated as ONPG) is a synthetic compound widely used as a substrate in biochemical assays, particularly in the study of enzyme activity, such as β-galactosidase. It is a colorless, crystalline solid that is soluble in water and organic solvents. ONPG is characterized by its ability to release o-nitrophenol upon hydrolysis, which can be quantitatively measured due to its yellow color, making it a useful indicator in enzyme assays. The compound features a thiogalactoside linkage, which is susceptible to enzymatic cleavage, allowing researchers to study the kinetics and mechanisms of glycosidase enzymes. Its molecular structure includes a nitrophenyl group, which contributes to its spectroscopic properties. ONPG is often utilized in microbiology for the detection of lactose fermentation and in molecular biology for the assessment of gene expression. Safety precautions should be observed when handling ONPG, as with many chemical substances, due to potential toxicity and environmental impact.

Formula: C₁₂H₁₅NO₇S

InChI: InChI=1/C12H15NO7S/c14-5-7-9(15)10(16)11(17)12(20-7)21-8-4-2-1-3-6(8)13(18)19/h1-4,7,9-12,14-17H,5H2

InChI key: InChIKey=SZAOZNVCHHBUDZ-RUXWNWLUSA-N

SMILES: S([C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)C2=C(N(=O)=O)C=CC=C2

Synonyms:

• 2-Nitrophenyl 1-Thiohexopyranoside
• 2-Nitrophenyl 1-thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• 2-Nitrophenyl-β-<span class="text-smallcaps">D</span>-thiogalactopyranoside
• 2-nitrophenyl 1-thio-beta-D-galactopyranoside
• Galactopyranoside, o-nitrophenyl 1-thio-
• Galactopyranoside, o-nitrophenyl 1-thio-, β-<span class="text-smallcaps">D</span>-
• Thio-o-nitrophenyl-β-<span class="text-smallcaps">D</span>-galactopyranoside
• beta-D-Galactopyranoside, 2-nitrophenyl 1-thio-
• o-Nitrophenol beta-thiogalactoside
• o-Nitrophenyl 1-thio-beta-D-galactopyranoside
• o-Nitrophenyl 1-thio-β-<span class="text-smallcaps">D</span>-galactoside
• o-Nitrophenyl β-<span class="text-smallcaps">D</span>-thiogalactoside
• β-<span class="text-smallcaps">D</span>-Galactopyranoside, 2-nitrophenyl 1-thio-
• Thio-o-nitrophenyl-β-D-galactopyranoside
• Galactopyranoside, o-nitrophenyl 1-thio-, β-D-
• 2-Nitrophenyl 1-thio-β-D-galactopyranoside
• β-D-Galactopyranoside, 2-nitrophenyl 1-thio-
• ONP-1-THIO-BETA-D-GAL
• O-NITROPHENYLSULFENYL-1-THIO-BETA-D-GALACTOSIDE
• 2-Nitrophenyl-beta-D-thiogalactopyranoside,98%
• o-Nitrophenylsulfenyl-1-thio-β-D-galactoside
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOSIDE
• 2-Nitrophenylb-D-thiogalactopyranoside
• 1-S-(2-Nitrophenyl)-1-thio-β-D-galactopyranose
• RARECHEM AH BS 0066
• 2-(hydroxymethyl)-6-[(2-nitrophenyl)thio]oxane-3,4,5-triol
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOPYRANOSIDE USP/EP/BP
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOPYRANOSID
• 1-(2-Nitrophenylthio)-1-deoxy-β-D-galactopyranose
• 2-NITROPHENYL-BETA-D-THIOGALACTOPYRANOSIDE
• O-NITROPHENYL-BETA-D-THIOGALACTOPYRANOSIDE
• O-NITROPHENYL 1-THIO-B-D-*GALACTOPYRANOS IDE
• 2-Nitrophenyl β-D-thiogalactopyranoside
• o-Nitrophenyl-1-thio-β-D-galactopyranoside≥ 99% (TLC)

β-D-Galactopyranoside, 2-nitrophenyl 1-thio-

CAS: 1158-17-4

Formula: C₁₂H₁₅NO₇S

Purity: 98%

Color and Shape: Solid

Molecular weight: 317.3150

Ref: IN-DA000GWC

2-Nitrophenyl b-D-thiogalactopyranoside

CAS: 1158-17-4

Ref: 7W-GC2622

2-Nitrophenyl 1-thio-β-D-galactopyranoside

CAS: 1158-17-4

2-Nitrophenyl 1-thio-β-D-galactopyranoside

Molecular weight: 317.32g/mol

Ref: 54-BIN1245

2-Nitrophenyl-β-D-thiogalactopyranoside

CAS: 1158-17-4

2-Nitrophenyl-beta-D-thiogalactopyranoside is a chromogenic substrate used to detect the presence of galactosidase enzymes in various biological and biochemical assays. It consists of a nitrophenyl group attached to a galactose sugar molecule through a thioglycosidic bond. When acted upon by enzymes such as galactosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry. This substrate is commonly used in assays for the detection of LacZ activity in bacterial cultures, as lacZ encodes for β-galactosidase.

Formula: C₁₂H₁₅NO₇S

Purity: Min. 98 Area-%

Color and Shape: Yellow Powder

Molecular weight: 317.32 g/mol

Ref: 3D-EN03315

2-Nitrophenyl-1-thio-β-D-galactopyranoside

CAS: 1158-17-4

2-Nitrophenyl-1-thio-beta-D-galactopyranoside is a nitroaryl compound that can be synthesized from beta-D-galactopyranosides and nitric acid. It has a molecular conformation that is stabilized by hydrogen bonds, which allows it to form supramolecular assemblies with other molecules. The nitro group in this molecule is responsible for the formation of the nitro group in 1,2,4,5,-tetranitrobenzene.

Formula: C₁₂H₁₅NO₇S

Molecular weight: 317.32 g/mol

Ref: 3D-N-5100