CAS 1170856-93-5

Calcein tetraethyl ester

Description:

Calcein tetraethyl ester is a fluorescent dye commonly used in biological and chemical applications, particularly in cell imaging and as a tracer in various assays. It is a non-toxic, cell-permeable compound that can easily penetrate cell membranes due to its ester groups. Once inside the cell, intracellular esterases hydrolyze the ester bonds, converting it into calcein, which is highly fluorescent and can be used to visualize live cells. The compound exhibits strong fluorescence in the green region of the spectrum, making it useful for various fluorescence microscopy techniques. Additionally, calcein tetraethyl ester is often employed in studies involving cell viability, apoptosis, and membrane integrity, as it can indicate live cells based on fluorescence intensity. Its stability and ease of use in aqueous solutions further enhance its utility in laboratory settings. Overall, calcein tetraethyl ester is a valuable tool in the fields of biochemistry and cell biology for studying cellular processes.

Tetraethyl 2,2',2'',2'''-(((3',6'-dihydroxy-3-oxo-3h-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetate

CAS: 1170856-93-5

Formula: C₃₈H₄₂N₂O₁₃

Purity: 99.52%

Color and Shape: Solid

Molecular weight: 734.7457

Ref: IN-DA000DO6

Tetraethyl 2,2',2'',2'''-(((3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetate

CAS: 1170856-93-5

Tetraethyl 2,2',2'',2'''-(((3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetate

Purity: 99%

Molecular weight: 734.75g/mol

Ref: 54-OR1066742

Calcein tetraethyl ester

CAS: 1170856-93-5

Calcein tetraethyl ester is a Calcein derivative and an intermediate in the synthesis of Calcein AM. Calcein is a fluorescent dye and self-quenching probe.

Formula: C₃₈H₄₂N₂O₁₃

Purity: 99.92%

Color and Shape: Solid

Molecular weight: 734.75

Ref: TM-T18932

Tetraethyl Fluorescein Bis(methylene)bis(azanetriyl)tetraacetate

Controlled Product

CAS: 1170856-93-5

Applications Tetraethyl Fluorescein Bis(methylene)bis(azanetriyl)tetraacetate is an intermediate used in the synthesis of Calcein AM (C125400), which is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP.
References Feller, N., et al.: Br. J. Cancer, 72, 543 (1995); Dhar, S., et al.: Eur. J. Pharmacol., 346, 315 (1998); Essodaigui, M., et al.: Biochemistry, 37, 2243 (1998); Lemasters, J.J., et al.: Biochim. Biophys. Acta., 1366,177 (1998);

Formula: C₃₈H₄₂N₂O₁₃

Color and Shape: Neat

Molecular weight: 734.75

Ref: TR-T292720