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CAS 1184-43-6

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Tetranitro Blue Tetrazolium

Description:
Tetranitro Blue Tetrazolium (TNBT) is a chemical compound commonly used as a redox indicator and in various biochemical assays. It is characterized by its vibrant blue color, which results from its reduction to formazan derivatives when it interacts with reducing agents. TNBT is a tetrazolium salt, and its structure includes four nitro groups attached to a tetrazolium ring, contributing to its stability and solubility in polar solvents. The compound is often employed in microbiological studies to assess cell viability, as living cells can reduce TNBT, leading to a color change that indicates metabolic activity. Additionally, TNBT is utilized in histological staining and as a component in various laboratory protocols, including those related to enzyme activity and oxidative stress. Safety considerations should be taken into account when handling TNBT, as it may pose health risks if ingested or inhaled. Overall, TNBT serves as a valuable tool in both research and clinical settings due to its distinctive properties and applications in detecting biological activity.
Formula:C40H28N12O10·2Cl
InChI:InChI=1S/C40H28N12O10.2ClH/c1-61-37-23-27(7-21-35(37)47-43-39(25-3-9-31(10-4-25)49(53)54)41-45(47)29-13-17-33(18-14-29)51(57)58)28-8-22-36(38(24-28)62-2)48-44-40(26-5-11-32(12-6-26)50(55)56)42-46(48)30-15-19-34(20-16-30)52(59)60;;/h3-24H,1-2H3;2*1H/q+2;;/p-2
InChI key:InChIKey=VCESGVLABVSDRO-UHFFFAOYSA-L
SMILES:O(C)C1=C(N2[N+](=NC(=N2)C3=CC=C(N(=O)=O)C=C3)C4=CC=C(N(=O)=O)C=C4)C=CC(=C1)C5=CC(OC)=C(C=C5)N6[N+](=NC(=N6)C7=CC=C(N(=O)=O)C=C7)C8=CC=C(N(=O)=O)C=C8.[Cl-]
Synonyms:
  • 2,2′,5,5′-Tetra-p-nitrophenyl-3,3′-(3,3′-dimethoxy-4,4′-biphenylene)ditetrazolium chloride
  • 2,2′,5,5′-Tetrakis(p-nitrophenyl)-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride
  • 2,2′,5,5′-Tetrakis-p-(nitrophenyl)-3,3′-bis(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride
  • 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(3,5-bis(4-nitrophenyl)-, chloride (1:2)
  • 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(3,5-bis(4-nitrophenyl)-, dichloride
  • 2H-Tetrazolium, 3,3′-(3,3′-dimethoxy-4,4′-biphenylylene)bis[2,5-bis(p-nitrophenyl)-, dichloride
  • 3,3'-(3,3'-Dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(2,5-bis(p-nitrophenyl)-2H-tetrazolium) dichloride
  • 3,3'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis[2,5-bis(4-nitrophenyl)-2H-tetrazol-3-ium]
  • 3,3'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis[2,5-bis(4-nitrophenyl)-2H-tetrazol-3-ium] dichloride
  • Blue p-nitrotetrazolium chloride
  • See more synonyms
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