CAS 1184-43-6

Tetranitro Blue Tetrazolium

Description:

Tetranitro Blue Tetrazolium (TNBT) is a chemical compound commonly used as a redox indicator and in various biochemical assays. It is characterized by its vibrant blue color, which results from its reduction to formazan derivatives when it interacts with reducing agents. TNBT is a tetrazolium salt, and its structure includes four nitro groups attached to a tetrazolium ring, contributing to its stability and solubility in polar solvents. The compound is often employed in microbiological studies to assess cell viability, as living cells can reduce TNBT, leading to a color change that indicates metabolic activity. Additionally, TNBT is utilized in histological staining and as a component in various laboratory protocols, including those related to enzyme activity and oxidative stress. Safety considerations should be taken into account when handling TNBT, as it may pose health risks if ingested or inhaled. Overall, TNBT serves as a valuable tool in both research and clinical settings due to its distinctive properties and applications in detecting biological activity.

Formula: C₄₀H₂₈N₁₂O₁₀·2Cl

InChI: InChI=1S/C40H28N12O10.2ClH/c1-61-37-23-27(7-21-35(37)47-43-39(25-3-9-31(10-4-25)49(53)54)41-45(47)29-13-17-33(18-14-29)51(57)58)28-8-22-36(38(24-28)62-2)48-44-40(26-5-11-32(12-6-26)50(55)56)42-46(48)30-15-19-34(20-16-30)52(59)60;;/h3-24H,1-2H3;2*1H/q+2;;/p-2

InChI key: InChIKey=VCESGVLABVSDRO-UHFFFAOYSA-L

SMILES: O(C)C1=C(N2[N+](=NC(=N2)C3=CC=C(N(=O)=O)C=C3)C4=CC=C(N(=O)=O)C=C4)C=CC(=C1)C5=CC(OC)=C(C=C5)N6[N+](=NC(=N6)C7=CC=C(N(=O)=O)C=C7)C8=CC=C(N(=O)=O)C=C8.[Cl-]

Synonyms:

• 2,2′,5,5′-Tetra-p-nitrophenyl-3,3′-(3,3′-dimethoxy-4,4′-biphenylene)ditetrazolium chloride
• 2,2′,5,5′-Tetrakis(p-nitrophenyl)-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride
• 2,2′,5,5′-Tetrakis-p-(nitrophenyl)-3,3′-bis(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride
• 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(3,5-bis(4-nitrophenyl)-, chloride (1:2)
• 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(3,5-bis(4-nitrophenyl)-, dichloride
• 2H-Tetrazolium, 3,3′-(3,3′-dimethoxy-4,4′-biphenylylene)bis[2,5-bis(p-nitrophenyl)-, dichloride
• 3,3'-(3,3'-Dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(2,5-bis(p-nitrophenyl)-2H-tetrazolium) dichloride
• 3,3'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis[2,5-bis(4-nitrophenyl)-2H-tetrazol-3-ium]
• 3,3'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis[2,5-bis(4-nitrophenyl)-2H-tetrazol-3-ium] dichloride
• Blue p-nitrotetrazolium chloride
• Nitroblue monotetrazolium chloride
• Nsc 121208
• Tetranitro Blue Tetrazolium
• Tetranitro Blue tetrazolium chloride hydrate
• Tetranitro-BT
• Tetranitroblue tetrazolium chloride
• Tetranitrotetrazolium blue
• Tnbt

Tetranitro blue tetrazolium chloride, 97%

CAS: 1184-43-6

Redox indicator for enzymes

Formula: C₄₀H₂₈Cl₂N₁₂O₁₀

Purity: 97%

Color and Shape: Yellow, Powder or crystals or crystalline powder

Molecular weight: 907.64

Ref: 02-J61080

Tetranitroblue tetrazolium chloride

CAS: 1184-43-6

Formula: C₄₀H₂₈Cl₂N₁₂O₁₀

Purity: 96%

Color and Shape: Solid

Molecular weight: 907.6307

Ref: IN-DA003UQU

Tetranitroblue tetrazolium chloride

CAS: 1184-43-6

Formula: C₄₀H₂₈Cl₂N₁₂O₁₀·2C₂H₆O

Purity: (HPLC) ≥ 96.0%

Color and Shape: Yellow crystalline powder

Molecular weight: 999.78

Ref: 7W-GK5496

Tetranitroblue tetrazolium chloride

CAS: 1184-43-6

Tetranitroblue tetrazolium chloride (TNBT) is a reagent that is used to measure the activity of enzymes in biological samples. TNBT can be used as a histological stain for myocardial infarcts and other areas of necrosis. It has been shown to be reactive with toll-like receptors, which are proteins that play a role in the immune response. The reaction mechanism of TNBT starts with its conversion to blue formazan crystals by peroxidase or other oxidizing enzymes. These crystals are reduced back to their original colorless form by reducing agents such as NADH or glutathione, which are present in most cells. This reduction process generates an electron that is then transferred to NAD+, forming NADH and releasing H2O2 as a byproduct.

Formula: C₄₀H₂₈Cl₂N₁₂O₁₀

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 907.63 g/mol

Ref: 3D-ET31974