CAS 124387-19-5

5(6)-carboxyfluorescein diacetate

Description:

5(6)-Carboxyfluorescein diacetate is a fluorescent dye commonly used in biological and biochemical applications, particularly for cell viability assays and as a tracer in various experimental setups. This compound is a non-fluorescent, membrane-permeable ester that, upon entering live cells, is hydrolyzed by intracellular esterases to release the fluorescent 5(6)-carboxyfluorescein. The resulting compound exhibits strong fluorescence, making it useful for detecting viable cells. Its characteristics include a high quantum yield and stability under physiological conditions, which enhances its utility in fluorescence microscopy and flow cytometry. The compound is typically characterized by its bright green fluorescence when excited by appropriate wavelengths. Additionally, it is important to note that the compound's fluorescence can be affected by pH and the presence of certain ions or molecules, which can influence its application in various experimental contexts. Overall, 5(6)-carboxyfluorescein diacetate serves as a valuable tool in cellular biology for assessing cell health and tracking cellular processes.

Formula: C₅₀H₃₂O₁₈

InChI: InChI=1/2C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25;1-12(26)31-14-6-8-18-20(10-14)33-21-11-15(32-13(2)27)7-9-19(21)25(18)22-16(23(28)29)4-3-5-17(22)24(30)34-25/h2*3-11H,1-2H3,(H,28,29)

SMILES: CC(=O)Oc1ccc2c(c1)Oc1cc(ccc1C12c2cc(ccc2C(=O)O1)C(=O)O)OC(=O)C.CC(=O)Oc1ccc2c(c1)Oc1cc(ccc1C12c2c(cccc2C(=O)O1)C(=O)O)OC(=O)C

Synonyms:

• 5(6)-Cfda
• 5(6)-Carboxy-Di-O-Acetylfluorescein
• 5(6)-Fam Diacetate
• 5-(And-6)-Carboxyfluorescein Diacetate
• Cfda
• Carboxyfluorescein Di-O-Acetate
• 5(6)-Carboxyfluorescein Diacetate, For F Luorescence
• 3',6'-Diacetoxy-1-Oxo-Spiro[Isobenzofuran-3,9'-Xanthene]-4-Carboxylic Acid
• 3',6'-Diacetoxy-1-Oxo-Spiro[Isobenzofuran-3,9'-Xanthene]-5-Carboxylic Acid
• -5(6)-CARBOXYFLUORESCEIN DIACETATE
• 90% (TLC)
• 5(6)-Carboxyfluorescein diacetate, for fluorescence, ≥90.0% (HPLC)
• 5-(and 6)-Carboxyfluorescein diacetate, mxed isomers
• Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylicacid, 3',6'-bis(acetyloxy)-3-oxo-
• 5(6)-FAM DA
• 5(6)-CFDA [5(6)-Carboxyfluorescein diacetate]
• Carboxyfluoresceindi-O-acetate(CFDA)

Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo-

CAS: 124387-19-5

Formula: C₅₀H₃₂O₁₈

Purity: 90%

Color and Shape: Solid

Molecular weight: 920.7783

Ref: IN-DA000LXX

5(6)-CFDA

CAS: 124387-19-5

5(6)-CFDA

Purity: ≥95%

Molecular weight: 920.78g/mol

Ref: 54-BUP11739

5(6)-CFDA

CAS: 124387-19-5

Ref: 7W-GW3794

5(6)-CFDA

CAS: 124387-19-5

5(6)-CFDA: cell-permeable, esterase-responsive viability probe; fluoresces upon activation, indicating enzymatic activity and membrane integrity.

Formula: C₅₀H₃₂O₁₈

Purity: 95.25%

Color and Shape: Solid

Molecular weight: 920.78

Ref: TM-T18894

5(6)-Carboxyfluorescein diacetate

CAS: 124387-19-5

5(6)-Carboxyfluorescein diacetate

Color and Shape: Solid

Molecular weight: 920.78g/mol

Ref: 54-BIC1066

5(6)-Carboxyfluorescein diacetate

Controlled Product

CAS: 124387-19-5

5(6)-carboxyfluorescein diacetate is an autophagy-inducing agent that has been shown to elicit a significant cytotoxic response in human leukemia cells. 5(6)-carboxyfluorescein diacetate has also been used to identify the presence of intracellular bacteria by labeling the cell wall. This compound is a fluorescent probe that binds to the cell membrane, and can be detected with optical sensors. 5(6)-carboxyfluorescein diacetate has also been shown to inhibit cancer growth in animal models, and is currently being evaluated for its potential use as an anticancer therapy.

Formula: C₂₅H₁₆O₉

Purity: Min. 98.0 Area-%

Molecular weight: 460.39 g/mol

Ref: 3D-C-1826