CAS 125328-76-9

5-BROMO-4-CHLORO-3-INDOXYL-1-ACETATE

Description:

5-Bromo-4-chloro-3-indoxyl-1-acetate is a synthetic organic compound primarily used as a chromogenic substrate in biochemical assays, particularly in the detection of β-galactosidase activity. This compound features a complex indole structure, which contributes to its unique reactivity and colorimetric properties. Upon enzymatic hydrolysis, it produces a colored product, making it valuable in molecular biology and microbiology for visualizing enzyme activity. The presence of bromine and chlorine substituents enhances its chemical stability and reactivity, allowing for specific interactions in biological systems. Additionally, the acetate group serves as a protective moiety, which is cleaved during the enzymatic reaction, further facilitating the detection process. Overall, 5-bromo-4-chloro-3-indoxyl-1-acetate is an important tool in research and diagnostic applications, providing a reliable method for studying enzyme kinetics and cellular processes.

Formula: C₁₀H₇BrClNO₂

InChI: InChI=1/C10H7BrClNO2/c1-5(14)13-4-8(15)9-7(13)3-2-6(11)10(9)12/h2-4,15H,1H3

SMILES: CC(=O)n1cc(c2c1ccc(c2Cl)Br)O

Synonyms:

• X-1-Acetate
• 1-acetyl-5-bromo-4-chloro-1H-indol-3-ol
• 5-Bromo-4-chloro-3-indolyl-1-acetate
• Ethanone, 1-(5-bromo-4-chloro-3-hydroxy-1H-indol-1-yl)-
• 5-BROMO-4-CHLORO-3-INDOXYL-1-ACETATE
• 5-BROMO-4-CHLORO-3-INDOLYL-N-ACETATE
• N-Acetyl-5-broMo-4-chloro-3-hydroxy-1H-indole, X-1 acetate
• 1-(5-broMo-4-chloro-3-hydroxyindol-1-yl)ethanone
• 1-acetyl-5-bromo-4-chloro-3-hydroxyindole
• 1H-Indol-3-ol, 1-acetyl-5-bromo-4-chloro-

5-BROMO-4-CHLORO-3-INDOXYL-1-ACETATE

CAS: 125328-76-9

Formula: C₁₀H₇BrClNO₂

Molecular weight: 288.5251

Ref: IN-DA000NIF

1-Acetyl-5-bromo-4-chloro-1H-indol-3-ol

CAS: 125328-76-9

Formula: C₁₀H₇BrClNO₂

Molecular weight: 288.52

Ref: 7W-GX6734

5-Bromo-4-chloro-3-indolyl-1-acetate

CAS: 125328-76-9

5-Bromo-4-chloro-3-indolyl-1-acetate

Molecular weight: 288.53g/mol

Ref: 54-BIB6004

5-Bromo-4-chloro-3-indoxyl-1-acetate

CAS: 125328-76-9

5-Bromo-4-chloro-3-indoxyl-1-acetate is a chromogenic substrate used to detect the specific enzymatic activity of esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl-1-acetate is used in bacterial esterase activity assays (e.g. Pseudomonas spp.).

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 288.52 g/mol

Ref: 3D-EB56757

5-Bromo-4-chloro-3-indoxyl-1-acetate

CAS: 125328-76-9

5-Bromo-4-chloro-3-indoxyl-1-acetate is used to diagnose bronchial asthma. This drug is a chemical that binds to antibodies and triggers the release of histamine from mast cells. 5-Bromo-4-chloro-3-indoxyl acetate has been shown to disrupt the autoantibodies that are responsible for type 1 diabetes in animal models. It has also been shown to be effective in the diagnosis of autoimmune diseases such as rheumatoid arthritis, lupus erythematosus, and systemic sclerosis. The administration of 5-bromo-4 chloro 3 indoxyl acetate has been shown to lead to an increase in levels of leukocyte antigen (CD45) on peripheral blood lymphocytes and symptom scores in adults with asthma.

Formula: C₁₀H₇BrClNO₂

Molecular weight: 288.53 g/mol

Ref: 3D-B-6000