H-Ala-AFC, or N-Acetyl-L-alanine-7-amido-4-methylcoumarin, is a synthetic compound commonly used in biochemical research, particularly in enzyme assays and peptide synthesis. It features an alanine residue linked to a fluorescent coumarin moiety, which allows for the monitoring of enzymatic activity through fluorescence. The compound is characterized by its ability to serve as a substrate for various proteases, enabling the study of enzyme kinetics and mechanisms. Its structure includes an amide bond, which contributes to its stability and reactivity in biological systems. H-Ala-AFC is typically soluble in organic solvents and has specific excitation and emission wavelengths that facilitate its detection in laboratory settings. The use of H-Ala-AFC in assays is advantageous due to its sensitivity and the ease with which fluorescence can be quantified, making it a valuable tool in the fields of biochemistry and molecular biology. Proper handling and storage conditions are essential to maintain its integrity and effectiveness in experimental applications.

H-Ala-AFC

126910-31-4: H-Ala-AFC, or N-Acetyl-L-alanine-7-amido-4-methylcoumarin, is a synthetic compound commonly used in biochemical research, particularly in enzyme assays and peptide synthesis. It features an alanine residue linked to a fluorescent coumarin moiety, which allows for the monitoring of enzymatic activity through fluorescence. The compound is characterized by its ability to serve as a substrate for various proteases, enabling the study of enzyme kinetics and mechanisms. Its structure includes an amide bond, which contributes to its stability and reactivity in biological systems. H-Ala-AFC is typically soluble in organic solvents and has specific excitation and emission wavelengths that facilitate its detection in laboratory settings. The use of H-Ala-AFC in assays is advantageous due to its sensitivity and the ease with which fluorescence can be quantified, making it a valuable tool in the fields of biochemistry and molecular biology. Proper handling and storage conditions are essential to maintain its integrity and effectiveness in experimental applications.

Fluorogenic substrate for microsomal alanine aminopeptidase, excitation at 400 nm, emission at 505 nm.

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CAS 126910-31-4

H-Ala-AFC

H-Ala-AFC

Ref: 01-4009776

H-Ala-AFC trifluoroacetate salt

Ref: 3D-FA110541