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CAS 127770-45-0

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5(6)-carboxy-2',7'-dichlorofluorescein diacetate

Description:
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate, commonly referred to as DCFDA, is a fluorescent dye used primarily in biological and chemical research. It is a derivative of fluorescein, characterized by its carboxyl and dichloro substitutions, which enhance its solubility and fluorescence properties. This compound is typically utilized as a probe for detecting reactive oxygen species (ROS) in cells, as it can permeate cell membranes and is converted into a fluorescent form upon deacetylation by intracellular esterases. The resulting fluorescence can be quantitatively measured, making it a valuable tool in studies of oxidative stress and cellular metabolism. DCFDA is generally non-toxic and can be used in live-cell imaging, allowing researchers to monitor dynamic biological processes in real-time. Its stability and sensitivity to environmental conditions, such as pH and temperature, are important considerations for experimental design. Overall, DCFDA serves as a crucial reagent in various fields, including cell biology, pharmacology, and toxicology.
Formula:C25H14Cl2O9
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