Suc-Ala-Ala-Pro-Arg-pNA, also known as Succinyl-Ala-Ala-Pro-Arg-p-nitroanilide, is a synthetic peptide substrate commonly used in biochemical research, particularly in studies involving proteolytic enzymes. This compound features a sequence of amino acids, including alanine, proline, and arginine, with a succinyl group at the N-terminus and a p-nitroanilide group at the C-terminus, which serves as a chromogenic reporter. The presence of the p-nitroanilide moiety allows for the detection of enzymatic activity through colorimetric assays, as the cleavage of the peptide bond by specific proteases releases the p-nitroaniline, resulting in a measurable color change. The compound is typically soluble in aqueous solutions and is stable under standard laboratory conditions. Its specificity and sensitivity make it a valuable tool for studying enzyme kinetics and the mechanisms of proteolytic activity. As with many peptides, care must be taken regarding storage and handling to maintain its integrity and functionality in experimental applications.

Suc-Ala-Ala-Pro-Arg-pNA

131068-47-8: Suc-Ala-Ala-Pro-Arg-pNA, also known as Succinyl-Ala-Ala-Pro-Arg-p-nitroanilide, is a synthetic peptide substrate commonly used in biochemical research, particularly in studies involving proteolytic enzymes. This compound features a sequence of amino acids, including alanine, proline, and arginine, with a succinyl group at the N-terminus and a p-nitroanilide group at the C-terminus, which serves as a chromogenic reporter. The presence of the p-nitroanilide moiety allows for the detection of enzymatic activity through colorimetric assays, as the cleavage of the peptide bond by specific proteases releases the p-nitroaniline, resulting in a measurable color change. The compound is typically soluble in aqueous solutions and is stable under standard laboratory conditions. Its specificity and sensitivity make it a valuable tool for studying enzyme kinetics and the mechanisms of proteolytic activity. As with many peptides, care must be taken regarding storage and handling to maintain its integrity and functionality in experimental applications.

Suc-AAPR-pNA, a substrate for wild-type and K188D/D189K trypsins (Km = 32.8 mM respectively 50.8 mM).

CAS 131068-47-8

Suc-Ala-Ala-Pro-Arg-pNA

Suc-Ala-Ala-Pro-Arg-pNA

Ref: 01-4017320