CAS 13146-93-5

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium

Description:

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium, commonly referred to as MTT, is a yellow, water-soluble compound widely used in biological assays, particularly in cell viability and proliferation studies. This compound is characterized by its ability to be reduced by viable cells to form a purple formazan product, which can be quantified spectrophotometrically. The presence of the thiazole ring contributes to its biological activity, while the diphenyl groups enhance its stability and solubility properties. MTT is non-toxic to cells at low concentrations, making it suitable for in vitro experiments. Its reduction is primarily facilitated by mitochondrial enzymes, allowing researchers to assess cell metabolic activity. The compound is sensitive to light and should be stored in a cool, dark place to maintain its integrity. Overall, MTT serves as a crucial tool in pharmacological and toxicological research, providing insights into cellular health and the effects of various treatments on cell viability.

Formula: C₁₈H₁₆N₅S

InChI: InChI=1S/C18H16N5S/c1-13-14(2)24-18(19-13)23-21-17(15-9-5-3-6-10-15)20-22(23)16-11-7-4-8-12-16/h3-12H,1-2H3/q+1

InChI key: InChIKey=FTZIQBGFCYJWKA-UHFFFAOYSA-N

SMILES: CC=1N=C(N2[N+](=NC(=N2)C3=CC=CC=C3)C4=CC=CC=C4)SC1C

Synonyms:

• 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium
• 2-(4,5-Dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium
• 2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-

2H-Tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-

CAS: 13146-93-5

Formula: C₁₈H₁₈N₅S

Molecular weight: 336.434

Ref: IN-DA000W8S