Biotin amidite, with the CAS number 135137-87-0, is a chemical compound commonly used in the field of biochemistry and molecular biology, particularly in the synthesis of oligonucleotides. It is a derivative of biotin, a water-soluble B-vitamin that plays a crucial role in various metabolic processes. Biotin amidite is characterized by its ability to form stable phosphoramidate linkages, which are essential for the incorporation of biotin into nucleic acid sequences. This compound typically appears as a white to off-white solid and is soluble in organic solvents. Its reactivity is primarily attributed to the amidite functional group, which allows for efficient coupling reactions during oligonucleotide synthesis. Biotin amidite is valued for its role in creating biotinylated oligonucleotides, facilitating applications such as gene expression studies, molecular probes, and affinity purification techniques. Proper handling and storage conditions are essential to maintain its stability and reactivity, as it can be sensitive to moisture and air.

BIOTIN AMIDITE

135137-87-0: Biotin amidite, with the CAS number 135137-87-0, is a chemical compound commonly used in the field of biochemistry and molecular biology, particularly in the synthesis of oligonucleotides. It is a derivative of biotin, a water-soluble B-vitamin that plays a crucial role in various metabolic processes. Biotin amidite is characterized by its ability to form stable phosphoramidate linkages, which are essential for the incorporation of biotin into nucleic acid sequences. This compound typically appears as a white to off-white solid and is soluble in organic solvents. Its reactivity is primarily attributed to the amidite functional group, which allows for efficient coupling reactions during oligonucleotide synthesis. Biotin amidite is valued for its role in creating biotinylated oligonucleotides, facilitating applications such as gene expression studies, molecular probes, and affinity purification techniques. Proper handling and storage conditions are essential to maintain its stability and reactivity, as it can be sensitive to moisture and air.

<p>Biotin phosphoramidite is a biotin-containing compound that is used as an extracellular probe for bacterial cells. It can be used to permeabilize cells, and it has been shown to have cytosolic uptake in various cell types. Biotin phosphoramidite binds to peptidoglycan in the cytosol of bacterial cells, and this binding induces internalization. This compound also has the ability to bind to peptidoglycan in the cytosol of eukaryotic cells, but does not induce internalization. Experiments with vibrio parahaemolyticus suggest that biotin phosphoramidite is a marginally effective inhibitor of this organism's uptake of unmodified peptidoglycan.</p>

CAS 135137-87-0

BIOTIN AMIDITE

5'-Biotin phosphoramidite

Ref: 3D-PB57507