CAS 142439-99-4

4-methylumbelliferyl-7-(6-sulfo-2-*acetamido-2-de

Description:

4-Methylumbelliferyl-7-(6-sulfo-2-acetamido-2-deoxy-α-D-glucopyranoside), commonly referred to as a fluorogenic substrate, is a chemical compound used primarily in biochemical assays, particularly for the detection of specific enzymes. This compound features a methylumbelliferyl moiety, which is known for its fluorescent properties, allowing for sensitive detection when cleaved by enzymes such as glycosidases. The presence of a sulfonate group enhances its solubility in aqueous solutions, making it suitable for various biological applications. Additionally, the acetamido group contributes to the stability and specificity of the substrate. The compound is typically utilized in research settings to study enzyme kinetics and activity, providing a valuable tool for understanding carbohydrate metabolism and related biochemical pathways. Its fluorescent nature allows for real-time monitoring of enzymatic reactions, making it an essential reagent in molecular biology and biochemistry. Proper handling and storage conditions are necessary to maintain its integrity and effectiveness in experimental applications.

Formula: C₁₈H₂₀NNaO₁₁S

InChI: InChI=1/C18H21NO11S.Na/c1-8-5-14(21)29-12-6-10(3-4-11(8)12)28-18-15(19-9(2)20)17(23)16(22)13(30-18)7-27-31(24,25)26;/h3-6,13,15-18,22-23H,7H2,1-2H3,(H,19,20)(H,24,25,26);/q;+1/p-1/t13-,15-,16-,17-,18-;/m1./s1

SMILES: Cc1cc(=O)oc2cc(ccc12)OC1C(C(C(C(COS(=O)(=O)O)O1)O)O)N=C(C)O.[Na]

Synonyms:

• sodium [(2R,3S,4R,5R,6S)-5-acetamido-3,4-dihydroxy-6-(4-methyl-2-oxo-chromen-7-yl)oxy-tetrahydropyran-2-yl]methyl sulfate

4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-glucopyranoside 6-sulphate sodium salt

CAS: 142439-99-4

Formula: C₁₈H₂₀NNaO₁₁S

Molecular weight: 481.40

Ref: 7W-GC5948

4-Methylumbelliferyl-2-acetamido-2-deoxy-6-sulphate-β-D-glucopyranoside sodium salt

CAS: 142439-99-4

4-Methylumbelliferyl-2-acetamido-2-deoxy-6-sulphate-β-D-glucopyranoside sodium salt

Formula: C₁₈H₂₀NO₁₁S·Na

Purity: 99% (by tlc) (Typical Value in Batch COA)

Color and Shape: off white powder

Molecular weight: 481.41g/mol

Ref: 54-BIM1189

4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide-6-sulfate, sodium salt

CAS: 142439-99-4

4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.

Formula: C₁₈H₂₀NNaO₁₁S

Purity: Min. 98.0 Area-%

Molecular weight: 481.41 g/mol

Ref: 3D-M-5505

4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-glucopyranoside-6-sulfate sodium salt

CAS: 142439-99-4

4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.

Formula: C₁₈H₂₀NO₁₁SNa

Purity: Min. 98 Area-%

Color and Shape: White Powder

Molecular weight: 481.41 g/mol

Ref: 3D-EM03271

4-Methylumbelliferyl 2-Acetamido-2-deoxy-b-D-glucopyranoside 6-Sulphate Sodium Salt

Controlled Product

CAS: 142439-99-4

Applications 4-Methylumbelliferyl 2-acetamido-2-deoxy-b-D-glucopyranoside 6-sulphate sodium salt is a fluorescent substrate for isoenzyme A of N-acetyl-β-D-glucosaminidase (Hexosaminidase A). It can also be used in biological study of characterization of the mutant β-subunit of β-hexosaminidase for dimer formation responsible for the adult form of Sandhoff disease with the motor neuron disease phenotype.
References Yamada, K., et al.: J Biochem, 153, 111 (2013)

Formula: C₁₈H₂₁NO₁₁S·Na

Color and Shape: Neat

Molecular weight: 482.41

Ref: TR-M337660