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CAS 144110-42-9

:

5-Bromo-6-chloro-3-indolylb-D-glucuronide

Description:
5-Bromo-6-chloro-3-indolyl β-D-glucuronide, commonly referred to as X-Gluc, is a synthetic chemical compound primarily used as a substrate for the detection of β-glucuronidase activity in various biological assays. This compound is characterized by its indole structure, which is substituted with bromine and chlorine atoms, contributing to its unique reactivity and solubility properties. X-Gluc is often employed in histochemical staining techniques, particularly in plant and microbial studies, to visualize gene expression and enzyme activity. Upon hydrolysis by β-glucuronidase, it produces a colored product, facilitating easy identification and quantification. The compound is generally stable under standard laboratory conditions but should be handled with care due to its halogenated nature, which may pose environmental and health risks. Its applications extend to molecular biology, where it aids in the study of gene regulation and enzyme function, making it a valuable tool in both research and diagnostic settings.
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