CAS 1461-15-0

Calcein

Description:

Calcein is a fluorescent dye commonly used in biological and chemical applications, particularly in microscopy and cell imaging. It is characterized by its bright green fluorescence, which is emitted when excited by specific wavelengths of light. The chemical structure of calcein includes multiple carboxyl groups, contributing to its solubility in water and making it suitable for various aqueous environments. Its molecular formula is C27H22N2O7, and it has a relatively high molar absorptivity, enhancing its utility in fluorescence-based assays. Calcein is often utilized as a viability stain in live/dead cell assays, as it can penetrate live cells and emit fluorescence, while non-viable cells do not retain the dye. Additionally, calcein can chelate metal ions, which allows it to be used in studies involving metal ion detection and quantification. Overall, calcein's stability, solubility, and fluorescent properties make it a valuable tool in both research and clinical settings.

Formula: C₃₀H₂₆N₂O₁₃

InChI: InChI=1S/C30H26N2O13/c33-21-7-23-19(5-15(21)9-31(11-25(35)36)12-26(37)38)30(18-4-2-1-3-17(18)29(43)45-30)20-6-16(22(34)8-24(20)44-23)10-32(13-27(39)40)14-28(41)42/h1-8,33-34H,9-14H2,(H,35,36)(H,37,38)(H,39,40)(H,41,42)

InChI key: InChIKey=DEGAKNSWVGKMLS-UHFFFAOYSA-N

SMILES: O=C1OC2(C=3C(OC=4C2=CC(CN(CC(O)=O)CC(O)=O)=C(O)C4)=CC(O)=C(CN(CC(O)=O)CC(O)=O)C3)C=5C1=CC=CC5

Synonyms:

• 2,2',2'',2'''-[(3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-2',7'-diyl)bis(methanediylnitrilo)]tetraacetic acid
• 2,2′,2′′,2′′′-(((3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-2′,7′-diyl)bis(methylene))bis(azanetriyl))tetraacetic acid
• 2,7-Bis[N,N-bis(carboxymethyl)aminomethylene]fluorescein
• 2-[[7′-[[Bis(carboxymethyl)amino]methyl]-3′,6′-dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-2′-yl]methyl-(carboxymethyl)amino]acetic acid
• Acetic acid, [(3′,6′-dihydroxy-2′,7′-fluorandiyl)bis(methylenenitrilo)]tetra-
• Fluorescein complexon
• Fluorescein, 2′,7′-bis[[bis(carboxymethyl)amino]methyl]-
• Fluorexon
• Glycine, N,N′-[(3′,6′-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-2′,7′-diyl)bis(methylene)]bis[N-(carboxymethyl)-
• N,N′-[(3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-2′,7′-diyl)bis(methylene)]bis[N-(carboxymethyl)glycine]
• NSC 298193
• Oftasceine

Calcein

CAS: 1461-15-0

Calcein is a fluorescent dye for detecting lipid vesicle leaks and calcium titration with EDTA.

Formula: C₃₀H₂₆N₂O₁₃

Purity: ≥98%

Color and Shape: Orange Powder

Molecular weight: 622.53

Ref: TM-T14855

N,N'-[(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-diyl)bis(methylene)]bis[N-(carboxymethyl)glycine]

CAS: 1461-15-0

Formula: C₃₀H₂₆N₂O₁₃

Color and Shape: Solid

Molecular weight: 622.5330

Ref: IN-DA001DOY

Calcein

CAS: 1461-15-0

Calcein

Purity: ≥98%

Molecular weight: 622.53g/mol

Ref: 54-BUP17456

Calcein (mixture of isomers) [for Fluorometric Determination of Ca]

CAS: 1461-15-0, 154071-48-4

Formula: C₃₀H₂₆N₂O₁₃

Color and Shape: Orange to Brown powder to crystaline

Molecular weight: 622.54

Ref: 3B-C0004

Fluorexon

CAS: 1461-15-0

Fluorexon is a novel fluorogenic probe that can be used as an optical sensor to measure the activity of polymerase chain reactions. Fluorexon has been shown to localize in mitochondria and to induce cytotoxicity in cancer cells. The drug also induces apoptosis through mitochondrial membrane potential depolarization and oxidative injury. This compound has demonstrated acute toxicities, but it is not known if these are due to its effects on drug transporters or its ability to increase cytosolic calcium.

Formula: C₃₀H₂₆N₂O₁₃

Color and Shape: Yellow Powder

Molecular weight: 622.53 g/mol

Ref: 3D-FF52656

Calcein

Controlled Product

CAS: 1461-15-0

Applications Calcein (cas# 1461-15-0) is a useful research chemical.

Formula: C₃₀H₂₆N₂O₁₃

Color and Shape: Neat

Molecular weight: 622.53

Ref: TR-C125413

Calcein Reagent extrapure AR (Fluorescein Complexone)

CAS: 1461-15-0

Formula: C₃₀H₂₆N₂O₁₃

Color and Shape: Yellow to orange, Crystalline powder

Molecular weight: 622.50

Ref: SR-65201

Calcein (mixture of isomers)

CAS: 1461-15-0

Formula: C₃₀H₂₆N₂O₁₃

Purity: 95.0%

Color and Shape: Solid, Yellow powder

Molecular weight: 622.539

Ref: 10-F079430