CAS 1492-30-4

p-Nitrophenyl palmitate

Description:

p-Nitrophenyl palmitate is an organic compound that serves as a substrate in biochemical assays, particularly for the study of lipases and esterases. It is an ester formed from palmitic acid and p-nitrophenol, characterized by its long hydrophobic fatty acid chain and a polar nitrophenyl group. This compound is typically a white to pale yellow solid at room temperature and is soluble in organic solvents like ethanol and acetone, but has limited solubility in water due to its hydrophobic nature. Upon hydrolysis, p-nitrophenyl palmitate releases p-nitrophenol, which can be quantified spectrophotometrically, making it useful for enzyme activity assays. The presence of the nitro group enhances the compound's reactivity and provides a chromogenic property, allowing for easy detection and measurement. Additionally, it is important to handle this compound with care, as p-nitrophenol is toxic and can pose health risks if ingested or inhaled.

Formula: C₂₂H₃₅NO₄

InChI: InChI=1S/C22H35NO4/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-22(24)27-21-18-16-20(17-19-21)23(25)26/h16-19H,2-15H2,1H3

InChI key: InChIKey=LVZSQWIWCANHPF-UHFFFAOYSA-N

SMILES: O(C(CCCCCCCCCCCCCCC)=O)C1=CC=C(N(=O)=O)C=C1

Synonyms:

• 4-Nitrophenol palmitate
• 4-Nitrophenyl hexadecanoate
• Hexadecanoic acid, 4-nitrophenyl ester
• Palmitic acid 4-nitrophenyl ester
• Palmitic acid, p-nitrophenyl ester
• p-Nitrophenyl hexadecanoate
• p-Nitrophenyl palmitate
• 4-Nitrophenyl palmitate

4-Nitrophenyl palmitate, 98+%

CAS: 1492-30-4

The activities of free and immobilized PPL were determined by spectrophotometric assay using 4-nitrophenyl palmitate (pNPP) as substrate. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the

Formula: C₂₂H₃₅NO₄

Purity: 98+%

Color and Shape: White to pale cream, Crystals or powder or crystalline powder

Molecular weight: 377.53

Ref: 02-L10896

4-Nitrophenyl palmitate

CAS: 1492-30-4

4-Nitrophenyl palmitate

Formula: C₂₂H₃₅NO₄

Purity: >97% (tlc) (Typical Value in Batch COA)

Color and Shape: off-white crystalline powder

Molecular weight: 377.52g/mol

Ref: 54-BIN0442

4-Nitrophenyl palmitate

CAS: 1492-30-4

Formula: C₂₂H₃₅NO₄

Purity: ≥ 97.0%

Color and Shape: Off-white to yellow crystalline powder

Molecular weight: 377.53

Ref: 7W-GK3996

4-Nitrophenyl palmitate

CAS: 1492-30-4

4-Nitrophenyl palmitate is a lipase-sensitive chromogenic substrate commonly used in the study of lipase and esterase activity. Its hydrolysis by lipases produces a yellow 4-nitrophenol, which can be easily detected spectrophotometrically, offering a quantitative measure of enzyme activity. This substrate is widely utilized in the screening of lipase-producing microorganisms and the optimization of enzyme-catalyzed reactions in various fields, including biotechnology and pharmaceutical research.

Formula: C₂₂H₃₅NO₄

Purity: Min. 95%

Color and Shape: Off-White Powder

Molecular weight: 377.52 g/mol

Ref: 3D-EN15588

4-Nitrophenyl palmitate

CAS: 1492-30-4

Formula: C₂₂H₃₅NO₄

Purity: 98%

Color and Shape: Solid

Molecular weight: 377.525

Ref: 10-F240253

4-Nitrophenyl Palmitate

CAS: 1492-30-4

4-Nitrophenyl palmitate serves as a colorimetric substrate for lipase and esterase, enabling the quantification of enzyme activity through the release and colorimetric detection of 4-nitrophenol at 410 nm following enzymatic hydrolysis. This compound facilitates the characterization of enzyme activity across diverse bacterial and mammalian sources, including Burkholderia and porcine pancreatic lipases.

Formula: C₂₂H₃₅NO₄

Color and Shape: Solid

Molecular weight: 377.525

Ref: TM-T85005

Hexadecanoic acid, 4-nitrophenyl ester

CAS: 1492-30-4

Formula: C₂₂H₃₅NO₄

Color and Shape: Solid

Molecular weight: 377.5176

Ref: IN-DA001LH1