CAS 149231-66-3

N-acetyl-tyr-val-ala-asp P-nitroanilide

Description:

N-acetyl-tyr-val-ala-asp P-nitroanilide is a synthetic peptide substrate commonly used in biochemical assays, particularly for the study of proteolytic enzymes. This compound features a sequence of amino acids, including N-acetyltyrosine, valine, alanine, and aspartic acid, linked to a P-nitroanilide moiety, which serves as a chromogenic indicator. The presence of the P-nitroanilide group allows for the detection of enzymatic activity through colorimetric changes upon cleavage by specific proteases. The N-acetyl group enhances the stability and solubility of the peptide in aqueous solutions. This compound is typically utilized in research settings to investigate enzyme kinetics and substrate specificity, providing insights into various biological processes. Its structural characteristics, including the arrangement of amino acids and the functional groups present, contribute to its reactivity and interaction with enzymes, making it a valuable tool in biochemical research. Safety data sheets should be consulted for handling and storage guidelines, as with any chemical substance.

Formula: C₂₉H₃₆N₆O₁₀

Synonyms:

• Ac-Tyr-Val-Ala-Asp-pNA
• Ac-Yvad-Pna

L-α-Asparagine, N-acetyl-L-tyrosyl-L-valyl-L-alanyl-N-(4-nitrophenyl)-

CAS: 149231-66-3

Formula: C₂₉H₃₆N₆O₁₀

Purity: 97%

Color and Shape: Solid

Molecular weight: 628.6303

Ref: IN-DA001LIA

Ac-YVAD-pNA

CAS: 149231-66-3

Ac-YVAD-pNA is a synthetic tetrapeptide inhibitor, which is primarily derived from specific peptide sequences designed to selectively inhibit cysteine protease activity. It acts by mimicking the natural substrates of the enzyme caspase-1 and competitively inhibiting its activity. The acetylated N-terminus (Ac-) and para-nitroanilide (pNA) group at the C-terminus facilitate the penetration across cell membranes and spectrophotometric measurement, respectively.Ac-YVAD-pNA is extensively utilized in biochemical research to investigate the role of caspase-1, which is a key player in inflammatory processes and apoptosis. By inhibiting caspase-1, researchers can study its involvement in the maturation of pro-inflammatory cytokines such as IL-1β and IL-18. The use of this inhibitor is crucial in elucidating the mechanisms of pyroptosis, a form of programmed cell death associated with inflammation. This tool aids in dissecting the pathways implicated in various diseases, including autoimmune disorders, neurodegenerative diseases, and infections, providing insights into potential therapeutic targets.

Formula: C₂₉H₃₆N₆O₁₀

Molecular weight: 628.63 g/mol

Ref: 3D-J-008571

Ac-YVAD-pNA

CAS: 149231-66-3

Ac-YVAD-pNA, a specific substrate for Caspase-1, serves to detect Caspase-1 activity, a crucial mediator of inflammatory processes [1] [2].

Formula: C₂₉H₃₆N₆O₁₀

Color and Shape: Solid

Molecular weight: 628.639

Ref: TM-T84839

Ac-Tyr-Val-Ala-Asp-pNA

CAS: 149231-66-3

Ac-YVAD-pNA, chromogenic substrate for caspase-1 (ICE).

Formula: C₂₉H₃₆N₆O₁₀

Purity: 98.7%

Color and Shape: Yellowish Powder

Molecular weight: 628.64

Ref: 01-4026201