Fluorescein diacetate 5-maleimide, with the CAS number 150322-01-3, is a fluorescent dye commonly used in biochemical applications, particularly in cell labeling and imaging. This compound is characterized by its ability to permeate cell membranes in its non-fluorescent diacetate form, where it is hydrolyzed by intracellular esterases to release fluorescein, a highly fluorescent compound. The maleimide group allows for specific conjugation to thiol-containing biomolecules, making it useful for targeted labeling of proteins and other macromolecules. The fluorescence properties of fluorescein diacetate 5-maleimide enable sensitive detection and visualization in various assays, including flow cytometry and microscopy. Additionally, its stability and compatibility with biological systems make it a valuable tool in molecular biology and biochemistry research. Overall, this compound serves as an important reagent for studying cellular processes and interactions in live cells.

fluorescein diacetate 5-maleimide

150322-01-3: Fluorescein diacetate 5-maleimide, with the CAS number 150322-01-3, is a fluorescent dye commonly used in biochemical applications, particularly in cell labeling and imaging. This compound is characterized by its ability to permeate cell membranes in its non-fluorescent diacetate form, where it is hydrolyzed by intracellular esterases to release fluorescein, a highly fluorescent compound. The maleimide group allows for specific conjugation to thiol-containing biomolecules, making it useful for targeted labeling of proteins and other macromolecules. The fluorescence properties of fluorescein diacetate 5-maleimide enable sensitive detection and visualization in various assays, including flow cytometry and microscopy. Additionally, its stability and compatibility with biological systems make it a valuable tool in molecular biology and biochemistry research. Overall, this compound serves as an important reagent for studying cellular processes and interactions in live cells.

Fluorescein diacetate 5-u200bmaleimide

<p>5-(N-Maleimido)fluorescein diacetate is a fluorescent probe that can be used to detect single-stranded DNA. This compound is not toxic to cells and has been shown to be a good indicator of the presence of double-stranded DNA. 5-(N-Maleimido)fluorescein diacetate is taken up by cells, where it binds to the dsDNA in the nucleus. The fluorescence is then detected using microscopy or flow cytometry. 5-(N-Maleimido)fluorescein diacetate can be used as a fluorescent probe for herpes simplex virus and other DNA viruses. It also binds to liposomes and has been shown to inhibit HIV replication in vitro and in vivo.</p>

CAS 150322-01-3

fluorescein diacetate 5-maleimide

Fluorescein diacetate 5-u200bmaleimide

Ref: 7W-GW4006

5-(N-Maleimido)fluorescein diacetate

Ref: 3D-FM52738