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CAS 150347-59-4

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5(6)-carboxyfluorescein diacetate N-succinimidyl este

Description:
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, commonly referred to as CFDA-SE, is a fluorescent dye used primarily in biological research for cell labeling and tracking. This compound is characterized by its ability to permeate cell membranes in its non-fluorescent diacetate form, where it is hydrolyzed by intracellular esterases to release the fluorescent carboxyfluorescein. The presence of the N-succinimidyl ester group enhances its reactivity with amine-containing biomolecules, facilitating covalent attachment to proteins or other cellular components. CFDA-SE exhibits strong fluorescence, making it suitable for applications in flow cytometry, microscopy, and live-cell imaging. Its stability and low toxicity further contribute to its utility in various experimental settings. The compound is typically used to study cell proliferation, migration, and differentiation, as it allows for the tracking of labeled cells over time. Overall, CFDA-SE is a valuable tool in cell biology and biochemistry for visualizing and quantifying cellular processes.
Formula:C29H19NO11
InChI:InChI=1/C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h3-8,11-13H,9-10H2,1-2H3
SMILES:CC(=O)Oc1ccc2c(c1)Oc1cc(ccc1C12c2ccc(cc2C(=O)O1)C(=O)ON1C(=O)CCC1=O)OC(=O)C
Synonyms:
  • 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester
  • 5-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate
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