CAS 150810-69-8

6-Carboxytetramethylrhodamine succinimidyl ester

Description:

6-Carboxytetramethylrhodamine succinimidyl ester (CAS 150810-69-8) is a fluorescent dye commonly used in bioconjugation and labeling applications in biochemical research. This compound features a rhodamine core, which is known for its strong fluorescence properties, making it suitable for various imaging techniques. The presence of a carboxyl group allows for further functionalization, while the succinimidyl ester moiety facilitates the conjugation to amine-containing biomolecules, such as proteins and peptides. This reactivity is particularly valuable in creating stable conjugates for use in assays, microscopy, and flow cytometry. The dye exhibits high quantum yield and photostability, which are essential for reliable fluorescence measurements. Additionally, its bright red fluorescence can be easily distinguished from other commonly used fluorophores, enabling multiplexing in experiments. Overall, 6-Carboxytetramethylrhodamine succinimidyl ester is a versatile tool in molecular biology for tracking and visualizing biomolecules in various experimental settings.

Formula: C₂₉H₂₅N₃O₇

InChI: InChI=1S/C29H25N3O7/c1-30(2)17-6-9-20-23(14-17)38-24-15-18(31(3)4)7-10-21(24)27(20)22-13-16(5-8-19(22)28(35)36)29(37)39-32-25(33)11-12-26(32)34/h5-10,13-15H,11-12H2,1-4H3

InChI key: InChIKey=PAOQTZWNYMMSEE-UHFFFAOYSA-N

SMILES: C([O-])(=O)C1=C(C=2C3=C([O+]=C4C2C=CC(N(C)C)=C4)C=C(N(C)C)C=C3)C=C(C(ON5C(=O)CCC5=O)=O)C=C1

Synonyms:

• 2-[3-(Dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]-4-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate
• 6-Carboxytetramethylrhodamine N-succinimidyl ester
• 6-Carboxytetramethylrhodamine, Succinimide Ester
• 6-TAMRA-NHS ester
• 6-Tamra Se
• Xanthylium, 9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-3,6-bis(dimethylamino)-, inner salt
• 6-CARBOXYTETRAMETHYLRHODAMINE, SUCCINIMIDYL ESTER

Xanthylium, 9-[2-carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-3,6-bis(dimethylamino)-, inner salt

CAS: 150810-69-8

Formula: C₂₉H₂₅N₃O₇

Purity: 97%

Color and Shape: Solid

Molecular weight: 527.5247

Ref: IN-DA001MVY

6-TAMRA-SE

CAS: 150810-69-8

6-TAMRA-SE

Purity: 95%

Molecular weight: 527.52g/mol

Ref: 54-OR1034737

6-TAMRA N-succinimidyl ester

CAS: 150810-69-8

Ref: 7W-GW0891

6-Carboxytetramethylrhodamine succinimidyl ester

CAS: 150810-69-8

6-Carboxytetramethylrhodamine (TAMRA) is a widely employed fluorescent dye (orange-red) suitable for different uses. In PCR applications, it has been used both as a reporter at the 5’ or as a quencher at the 3’ end. TAMRA-labelled oligonucleotides have been used to study interactions with DNA or DNA/proteins interactions. It is an excellent fluorescent acceptor for FRET applications with a suitable dye pair. It is used as a protein/antibody labelling agent for structural studies, immunochemistry and flow cytometry. Bioconjugation is generally performed through coupling of the carboxylic acid to a primary amine at the N-terminus of a protein, on lysine side chains or amino-modified oligonucleotide. Carboxylic acids activated with succinimidyl esters react with primary amines.

Formula: C₂₉H₂₅N₃O₇

Purity: Min. 95 Area-%

Color and Shape: Powder

Molecular weight: 527.52 g/mol

Ref: 3D-FC45650

6-TAMRA-SE

CAS: 150810-69-8

6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine and one of the traditional fluorophores used for automated DNA sequencing.

Formula: C₂₉H₂₅N₃O₇

Purity: 98%

Color and Shape: Solid

Molecular weight: 527.52

Ref: TM-T18904