CAS 152957-34-1

O-nitrophenyl-N-acetyl-B-D-*galactosaminide

Description:

O-nitrophenyl-N-acetyl-B-D-galactosaminide is a synthetic compound commonly used as a substrate in biochemical assays, particularly for the detection of specific glycosidase enzymes. This compound features a nitrophenyl group, which provides a chromogenic property, allowing for colorimetric detection upon enzymatic hydrolysis. The N-acetyl-B-D-galactosaminide moiety is a derivative of galactosamine, a sugar amino acid, which is significant in various biological processes. The presence of the nitro group enhances the compound's solubility and reactivity, making it suitable for laboratory applications. It is typically used in research settings to study enzyme kinetics and carbohydrate metabolism. Safety data sheets indicate that, like many organic compounds, it should be handled with care, as it may pose health risks if ingested or inhaled. Overall, O-nitrophenyl-N-acetyl-B-D-galactosaminide serves as an important tool in enzymology and carbohydrate chemistry.

Formula: C₁₄H₁₈N₂O₈

2-Nitrophenyl 2-acetamido-2-deoxy-b-D-galactopyranose

CAS: 152957-34-1

Ref: 7W-GC4565

2-Nitrophenyl 2-acetamido-2-deoxy-b-D-galactopyranoside

CAS: 152957-34-1

2-Nitrophenyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a carboxymethyl derivative of the natural substrate of β-galactosidase, which is used as a substrate for the production of recombinant proteins in the corynebacterium glutamicum. The enzyme β-galactosidase hydrolyzes this substrate to produce an acid molecule and a sugar molecule. This product can be used in biotechnological processes for the production of recombinant proteins or other bioactive molecules. It can also be used in the isolation of nucleic acid from bacteria or fungi, or to study bacterial membrane transport systems. 2NPGAG can be expressed as a recombinant protein with expression vectors such as pET21a and pET24a. It has been shown that this product inhibits transport through membranes by binding to cellulose, which may be useful for studies on cellulose biosynthesis

Purity: Min. 95%

Molecular weight: 342.3 g/mol

Ref: 3D-EN05078