CAS 153248-58-9

N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]-6'-O-methylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite

Description:

N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]-6'-O-methylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is a complex nucleoside phosphoramidite used primarily in the synthesis of oligonucleotides. This compound features several key functional groups, including a phosphoramidite moiety, which is crucial for the formation of phosphodiester bonds during oligonucleotide synthesis. The presence of protective groups, such as the dimethoxytrityl and tert-butyl dimethylsilyl groups, indicates that this compound is designed to prevent unwanted reactions during the synthesis process. The acetyl and methyl modifications on the guanosine base enhance its stability and solubility. The cyanoethyl group is important for the activation of the phosphoramidite, facilitating the coupling reaction with the growing oligonucleotide chain. Overall, this compound is characterized by its high reactivity, stability under appropriate conditions, and specificity for use in the synthesis of RNA and DNA sequences, making it a valuable tool in molecular biology and genetic research.

Formula: C₄₉H₆₆N₇O₉PSi

Synonyms:

• 2’-TBDMS-rG(N-Ac) Phosphoramidite
• 5'-O-DMT-2'-TBDMS-6'-O-Me-rG(N-Ac)-3'-CEDPA

N-Acetyl-2'-O-[(tert-butyl)dimethylsilyl]-5'-O-DMT-6'-O-methylguanosine-3'-CE phosphoramidite

CAS: 153248-58-9

N-Acetyl-2'-O-[(tert-butyl)dimethylsilyl]-5'-O-DMT-6'-O-methylguanosine-3'-CE phosphoramidite is a nucleoside and nucleotide analog. It is synthesized by reacting 2'-deoxyguanosine with acetyl chloride, 5'-dimethoxytrityl chloride, and 3'-Ce phosphoramidite in an organic solvent. N-Acetyl-2'-O-[(tert-butyl)dimethylsilyl]-5'-O-DMT-6'-O-methylguanosine has been shown to inhibit the growth of cancer cells and viruses in cell culture studies. It is also a potent inhibitor of HIV replication in vitro.

Formula: C₄₉H₆₆N₇O₉PSi

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 956.15 g/mol

Ref: 3D-PA152483