Description:HEPBS, or 2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid, is a zwitterionic buffering agent commonly used in biological and biochemical applications. It is characterized by its ability to maintain a stable pH in solutions, making it particularly useful in cell culture and various laboratory experiments. HEPBS is soluble in water and exhibits low toxicity, which makes it suitable for use in sensitive biological systems. The compound has a pKa value that allows it to effectively buffer physiological pH ranges, typically around neutral pH. Its structure includes a piperazine ring, which contributes to its buffering capacity, and a sulfonic acid group that enhances its solubility. HEPBS is often preferred over other buffers due to its minimal interference with biological processes and its stability under a range of conditions. Overall, HEPBS is a valuable tool in research and industrial applications where precise pH control is essential.
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