CAS 162758-04-5

N-Biotinylaminoethyl Methanethiosulfonate

Description:

N-Biotinylaminoethyl Methanethiosulfonate, with the CAS number 162758-04-5, is a chemical compound that serves as a biotinylation reagent, commonly used in biochemical and molecular biology applications. This compound features a biotin moiety, which is a vitamin that plays a crucial role in cellular metabolism and is widely utilized for tagging proteins and other biomolecules. The methanethiosulfonate group in its structure allows for selective conjugation to thiol groups, facilitating the attachment of biotin to proteins or other molecules containing cysteine residues. This property is particularly valuable in affinity purification and detection methods, such as streptavidin-biotin interactions, which are characterized by their high specificity and strong binding affinity. N-Biotinylaminoethyl Methanethiosulfonate is typically used in research settings to study protein interactions, localization, and function, making it an essential tool in proteomics and related fields. Its stability and reactivity under physiological conditions further enhance its utility in various experimental protocols.

Formula: C₁₃H₂₃N₃O₄S₃

InChI: InChI=1/C13H23N3O4S3/c1-23(19,20)22-7-6-14-11(17)5-3-2-4-10-12-9(8-21-10)15-13(18)16-12/h9-10,12H,2-8H2,1H3,(H,14,17)(H2,15,16,18)/t9?,10-,12?/m0/s1

SMILES: CS(=O)(=O)SCCN=C(CCCC[C@H]1C2C(CS1)N=C(N2)O)O

O-(2-(5-((3AS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethyl) methanesulfonothioate

CAS: 162758-04-5

Formula: C₁₃H₂₃N₃O₄S₃

Color and Shape: Solid

Molecular weight: 381.5344

Ref: IN-DA001TE8

N-Biotinylaminoethyl methylthiosulphonate

CAS: 162758-04-5

N-Biotinylaminoethyl methylthiosulphonate

Purity: ≥95%

Molecular weight: 381.53g/mol

Ref: 54-OR9475T

N-Biotinylaminoethyl Methanethiosulfonate

Controlled Product

CAS: 162758-04-5

Stability Moisture Sensitive
Applications Reacts specifically and rapidly with thiols to form mixed disulfides. Used to probe the structures of the ACh receptor channel of the GABA receptor channel and of lactose permease. MTSEA-biotin has recently been used to probe the topology of the serotonin (5-HT) transporter by site-directed chemical labelling.
References J. Biol. Chem., 273(20), 12675 (1998), Adams, C.M., et al.: J. Biol. Chem., 273 (46), 30204 (1998), Boileau, A. J., et al.: J. Neurosci., 19, 12, 4847 (1999), Wagner, D.A.and Czajkowski, C.: J. Neurosci., 21, 67 (2001), Traebert, M.: J. Membr. Biol., 180, 83 (2001)

Formula: C₁₃H₂₃N₃O₄S₃

Color and Shape: Neat

Molecular weight: 381.53

Ref: TR-B394750