CAS 165174-58-3

N-succinyl-ala-ala-pro-asp*P-nitroanilide

Description:

N-succinyl-ala-ala-pro-asp*P-nitroanilide, with the CAS number 165174-58-3, is a synthetic peptide substrate commonly used in biochemical research, particularly in studies involving proteolytic enzymes. This compound features a succinyl group that enhances its solubility and stability, making it suitable for various assays. The peptide sequence includes alanine (ala), proline (pro), and aspartic acid (asp), which are amino acids that contribute to its structural properties and reactivity. The presence of the p-nitroanilide moiety is significant as it serves as a chromogenic group, allowing for the quantification of enzymatic activity through colorimetric changes upon cleavage by specific proteases. This substrate is particularly valuable in the study of serine proteases and other related enzymes, providing insights into their kinetics and mechanisms. Overall, N-succinyl-ala-ala-pro-asp*P-nitroanilide is an important tool in biochemical assays, facilitating the understanding of enzyme function and regulation.

Formula: C₂₅H₃₂N₆O₁₁

Suc-Ala-Ala-Pro-Asp-pNA

CAS: 165174-58-3

Suc-Ala-Ala-Pro-Asp-pNA is a synthetic substrate that has been expressed, synthesized, and purified. It is hydrophobic with a phenylmethyl residue that is reactive. Suc-Ala-Ala-Pro-Asp-pNA has been shown to have protease activity in plate assays against serine proteases and has an acidic ph optimum. Suc-Ala-Ala-Pro-Asp-pNA also has an ionizable side chain at the reactive site and is thermostable.

Formula: C₂₅H₃₂N₆O₁₁

Purity: Min. 95%

Molecular weight: 592.56 g/mol

Ref: 3D-FS110787