CAS 16934-09-1

5-Bromo-3-indolyl-beta-D-glucopyranoside

Description:

5-Bromo-3-indolyl-beta-D-glucopyranoside, commonly referred to as X-Gluc, is a synthetic compound widely used as a substrate for the enzyme β-glucuronidase in molecular biology and histochemistry. This compound is characterized by its indole structure, which is a bicyclic compound consisting of a benzene ring fused to a pyrrole ring, and a glucopyranoside moiety that enhances its solubility in biological systems. The presence of the bromine atom at the 5-position of the indole ring contributes to its unique reactivity and makes it a useful tool for detecting β-glucuronidase activity in various biological samples. Upon enzymatic hydrolysis, X-Gluc produces a blue precipitate, allowing for visual identification of enzyme activity. This compound is often utilized in plant biology, particularly in studies involving gene expression and reporter assays. Its stability and specificity make it a valuable reagent in both research and diagnostic applications. Safety precautions should be observed when handling this compound, as with all chemical substances.

Formula: C₁₄H₁₆BrNO₆

InChI: InChI=1/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m0/s1

Synonyms:

• 5-Bromo-1H-indol-3-yl β-L-galactopyranoside
• β-L-galactopyranoside, 5-bromo-1H-indol-3-yl
• (2R,3S,4R,5S,6S)-2-[(5-bromo-1H-indol-3-yl)oxy]-6-(hydroxymethyl)tetrahydropyran-3,4,5-triol

5-Bromo-3-indolyl b-D-glucopyranoside

CAS: 16934-09-1

Formula: C₁₄H₁₆BrNO₆

Molecular weight: 374.20

Ref: 7W-GC1579

5-Bromo-3-indolyl β-D-glucopyranoside

CAS: 16934-09-1

5-Bromo-3-indolyl β-D-glucopyranoside

Formula: C₁₄H₁₆BrNO₆

Purity: By hplc: >97% (Typical Value in Batch COA)

Color and Shape: white powder

Molecular weight: 374.18g/mol

Ref: 54-BIB1406

5-Bromo-3-indolyl b-D-glucopyranoside

CAS: 16934-09-1

5-bromo-3-indolyl-b-d-glucopyranoside, also known as 5BIG, is an enzyme substrate for beta-glucosidase. It is used in bacterial assays for the identifcation of Gram-positive bacteria such as Staphylococcus aureus and Bacillus subtilis. After cleavege, it gives an intense blue color.

Formula: C₁₄H₁₆BrNO₆

Purity: Min. 98 Area-%

Color and Shape: Powder

Molecular weight: 374.18 g/mol

Ref: 3D-EB06829