CAS 191223-35-5

N-D-Biotinyl-7-amino-4-methylcoumarin

Description:

N-D-Biotinyl-7-amino-4-methylcoumarin is a biotinylated derivative of 7-amino-4-methylcoumarin, which is a fluorescent compound often used in biochemical assays and research. This substance features a biotin moiety, which allows for strong binding to avidin or streptavidin, facilitating its use in various applications such as protein labeling, detection, and purification. The coumarin structure contributes to its fluorescent properties, making it useful for imaging and tracking in biological systems. The compound is typically characterized by its solubility in organic solvents and its stability under physiological conditions, although specific stability can depend on the environment and conditions of use. Its fluorescence can be excited by specific wavelengths, allowing for sensitive detection in assays. Overall, N-D-Biotinyl-7-amino-4-methylcoumarin serves as a valuable tool in molecular biology and biochemistry, particularly in studies involving protein interactions and cellular processes.

Formula: C₂₀H₂₃N₃O₄S

Synonyms:

• (3aS,4S,6aR)-Hexahydro-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide

1H-Thieno[3,4-d]imidazole-4-pentanamide, hexahydro-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-2-oxo-, (3aS,4S,6aR)-

CAS: 191223-35-5

Formula: C₂₀H₂₃N₃O₄S

Color and Shape: Solid

Molecular weight: 401.4793

Ref: IN-DA002F4T

N-d-Biotinyl-7-amino-4-methylcoumarin

Controlled Product

CAS: 191223-35-5

Applications A novel fluorigenic substrate for the determination of Biotinidase activity.
References Kumugi, S., et al.: Chemistry Letters, 391 (1997)

Formula: C₂₀H₂₃N₃O₄S

Color and Shape: Neat

Molecular weight: 401.48

Ref: TR-B394925

N-D-Biotinyl-7-amino-4-methylcoumarin

CAS: 191223-35-5

N-D-Biotinyl-7-amino-4-methylcoumarin is a fluorescent substrate that is used to measure the activity of biotinidase, an enzyme that catalyzes the hydrolysis of biotin. It is detectable by measuring fluorescence with a spectrophotometer. Biotinidase deficiency can be diagnosed by measuring the activity of this enzyme in biological fluids. This assay has been shown to be useful for diagnosing patients with deficient biotinidase activity.

Formula: C₂₀H₂₃N₃O₄S

Purity: Min. 98 Area-%

Color and Shape: Powder

Molecular weight: 401.48 g/mol

Ref: 3D-FB152327