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CAS 200422-18-0

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4-nitrophenyl-β-D-galactopyranoside

Description:
4-Nitrophenyl-β-D-galactopyranoside is a synthetic compound commonly used as a substrate in biochemical assays, particularly for the detection of β-galactosidase activity. This compound features a galactopyranoside moiety linked to a 4-nitrophenyl group, which provides a chromogenic response upon hydrolysis. When β-galactosidase catalyzes the cleavage of the glycosidic bond, it releases 4-nitrophenol, resulting in a color change that can be quantitatively measured, making it a valuable tool in molecular biology and enzymology. The compound is typically a white to pale yellow solid and is soluble in water and organic solvents, facilitating its use in various laboratory settings. Its molecular structure includes a β-D-galactopyranoside unit, which is a six-membered cyclic form of galactose, contributing to its reactivity with specific enzymes. Safety data should be consulted, as with any chemical, to ensure proper handling and disposal.
Formula:C12H15NO8
InChI:InChI=1/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-6(2-4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8?,9-,10+,11?,12+/m0/s1
Synonyms:
  • 4-Nitrophenyl-beta-D-galactopyranoside
  • p-Nitrophenyl-β-D-Galactopyranoside
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