Description:H-D-Ala-AMC?TFA, with the CAS number 201847-52-1, is a chemical compound that typically functions as a peptide substrate in biochemical assays. It is a derivative of alanine, where "H-D-Ala" indicates the presence of a D-alanine residue, and "AMC" refers to 7-amino-4-methylcoumarin, a fluorescent moiety often used in enzymatic activity studies. The "TFA" denotes trifluoroacetic acid, which is commonly used in peptide synthesis and purification processes. This compound is characterized by its ability to release the fluorescent AMC upon cleavage by specific proteases, making it valuable for monitoring enzyme activity in various biological research applications. Its stability, solubility in organic solvents, and fluorescence properties make it suitable for high-throughput screening and kinetic studies. Overall, H-D-Ala-AMC?TFA serves as an important tool in the field of biochemistry and molecular biology for studying proteolytic enzymes and their mechanisms.
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