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CAS 214491-43-7

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6,8-Difluoro-4-methylumbelliferyl phosphate

Description:
6,8-Difluoro-4-methylumbelliferyl phosphate is a synthetic chemical compound primarily used as a substrate in biochemical assays, particularly for the detection of phosphatase activity. This compound features a fluorinated umbelliferone structure, which enhances its fluorescent properties, making it useful in various analytical applications. The presence of two fluorine atoms at the 6 and 8 positions of the umbelliferyl moiety increases the compound's stability and alters its electronic properties, contributing to its fluorescence characteristics. The phosphate group is crucial for its reactivity, allowing it to be hydrolyzed by phosphatases, resulting in the release of the fluorescent umbelliferone, which can be quantitatively measured. This compound is often utilized in molecular biology and biochemistry for enzyme assays, providing a sensitive and specific method for studying enzyme kinetics and activity. Safety data sheets should be consulted for handling and storage guidelines, as with any chemical substance, to ensure proper laboratory practices.
Formula:C10H7F2O6P
InChI:InChI=1S/C10H7F2O6P/c1-4-2-7(13)17-9-5(4)3-6(11)10(8(9)12)18-19(14,15)16/h2-3H,1H3,(H2,14,15,16)
InChI key:InChIKey=DZANYXOTJVLAEE-UHFFFAOYSA-N
SMILES:FC1=C2C(=CC(F)=C1OP(=O)(O)O)C(C)=CC(=O)O2
Synonyms:
  • DiFMUP
  • 2H-1-Benzopyran-2-one, 6,8-difluoro-4-methyl-7-(phosphonooxy)-
  • 6,8-Difluoro-4-methyl-7-(phosphonooxy)-2H-1-benzopyran-2-one
  • 6,8-Difluoro-4-methylumbelliferyl phosphate
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