CAS 216699-35-3

5-Carboxy-X-rhodamine

Description:

5-Carboxy-X-rhodamine, identified by its CAS number 216699-35-3, is a fluorescent dye commonly used in various biochemical and molecular biology applications. This compound is characterized by its strong fluorescence properties, which make it suitable for labeling and tracking biomolecules in live cells and tissues. The presence of a carboxylic acid group enhances its solubility in aqueous solutions, facilitating its use in biological systems. The dye exhibits a high extinction coefficient and quantum yield, contributing to its effectiveness in fluorescence microscopy and flow cytometry. Additionally, 5-Carboxy-X-rhodamine can be conjugated to proteins, nucleic acids, or other biomolecules, allowing for versatile applications in imaging and detection. Its stability under physiological conditions and compatibility with various labeling techniques further enhance its utility in research. Overall, 5-Carboxy-X-rhodamine is a valuable tool in the study of cellular processes, gene expression, and protein interactions.

Formula: C₃₃H₃₀N₂O₅

InChI: InChI=1S/C33H30N2O5/c36-32(37)20-9-10-21(24(17-20)33(38)39)27-25-15-18-5-1-11-34-13-3-7-22(28(18)34)30(25)40-31-23-8-4-14-35-12-2-6-19(29(23)35)16-26(27)31/h9-10,15-17H,1-8,11-14H2,(H-,36,37,38,39)

InChI key: InChIKey=UNGMOMJDNDFGJG-UHFFFAOYSA-N

SMILES: C([O-])(=O)C1=C(C=2C=3C(=C4C5=C(C3)CCCN5CCC4)[O+]=C6C2C=C7C8=C6CCCN8CCC7)C=CC(C(O)=O)=C1

Synonyms:

• 1H,5H,11H,15H-Xantheno[2,3,4-ij:5,6,7-i'j′]diquinolizin-18-ium, 9-(2,4-dicarboxyphenyl)-2,3,6,7,12,13,16,17-octahydro-, inner salt
• 5-Rox
• Red 5-ROX
• 5-Carboxy-X-rhodamine

5-Carboxy-x-rhodamine triethylamin salt

CAS: 216699-35-3

Formula: C₃₃H₃₀N₂O₅

Purity: 95%

Color and Shape: Solid

Molecular weight: 534.6017

Ref: IN-DA00BF5D

5-Carboxy-X-Rhodamine

CAS: 216699-35-3

5-Carboxy-X-Rhodamine

Purity: 98%

Molecular weight: 534.60g/mol

Ref: 54-OR1011842

5-ROX

CAS: 216699-35-3

5-ROX

Purity: ≥98%

Molecular weight: 534.6g/mol

Ref: 54-BUP07161

5-Carboxy-X-rhodamine

CAS: 216699-35-3

Formula: C₃₃H₃₀N₂O₅

Purity: >95.0%(HPLC)

Color and Shape: Gray to Dark purple to Black powder to crystal

Molecular weight: 534.61

Ref: 3B-C3538

5-ROX

CAS: 216699-35-3

Ref: 7W-GW2330

5-ROX

CAS: 216699-35-3

5-ROX is a bright rhodamine dye, fluoresces in water at 580 nm excitation, 604 nm emission, ε 3.6×10^4 M^−1cm^−1, ϕ 0.94.

Formula: C₃₃H₃₀N₂O₅

Purity: 98.85%

Color and Shape: Solid

Molecular weight: 534.6

Ref: TM-T18890

5-Carboxy-X-rhodamine

CAS: 216699-35-3

Single isomer of 5(6)-ROX is a fluorescent dye used in RT-PCR methods as an internal reference to determine fluorescence variation that is not associated with the amplification process (plastic of the wells, small differences in concentration or volume, instrument measurements). It produces a constant fluorescence emission signal during the PCR process that is used to normalise the emission produced by the reporter. The fluorescence signal is compatible with that of most reporters. It is used to label the 5’ end of oligonucleotides as a reporter in the presence of a quencher at the 3’ end (dual labelled probe). During the amplification process, the dye is cleaved, and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. 6-ROX with NHS-activated carboxylic acids reacts with primary amines.

Formula: C₃₃H₃₀N₂O₅

Purity: Min. 95%

Color and Shape: Powder

Molecular weight: 534.6 g/mol

Ref: 3D-FC72889

5-ROX

Controlled Product

CAS: 216699-35-3

Applications 5-ROX is a purified isomer of carboxy-X-rhodamine. 5-ROX is widely used for oligonucleotide labeling and automated DNA sequencing applications.

Formula: C₃₃H₂₉N₂NaO₅

Color and Shape: Neat

Molecular weight: 556.583

Ref: TR-R637385