CAS 219137-91-4

Z-Val-Ala-Asp-AMC

Description:

Z-Val-Ala-Asp-AMC, also known by its CAS number 219137-91-4, is a synthetic peptide substrate commonly used in biochemical research, particularly in the study of proteases. This compound features a Z (benzyloxycarbonyl) protecting group on the N-terminus, which enhances its stability and solubility. The sequence includes the amino acids valine (Val), alanine (Ala), and aspartic acid (Asp), followed by the AMC (7-amino-4-methylcoumarin) moiety, which serves as a fluorescent reporter. Upon cleavage by specific proteases, the AMC group is released, resulting in a measurable increase in fluorescence, making it a valuable tool for monitoring proteolytic activity in various assays. Z-Val-Ala-Asp-AMC is typically utilized in enzyme kinetics studies, drug discovery, and the characterization of protease inhibitors. Its characteristics include high specificity for certain proteases, good solubility in aqueous solutions, and stability under physiological conditions, which facilitate its application in both in vitro and in vivo studies.

Formula: C₃₀H₃₄N₄O₉

Synonyms:

• Z-Vad-Amc
• L-a-Asparagine, N-[(phenylmethoxy)carbonyl]-L-valyl-L-alanyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-

Z-Val-Ala-Asp-AMC

CAS: 219137-91-4

The fluorogenic substrate Z-VAD-AMC is cleaved by caspase-1 to generate free AMC. The cleavage was monitored by using an excitation wavelength of 365 nm and determining the emission at 450 nm.

Formula: C₃₀H₃₄N₄O₉

Purity: 99.2%

Color and Shape: White Powder

Molecular weight: 594.62

Ref: 01-4027536

Z-Val-Ala-Asp-AMC

CAS: 219137-91-4

Z-Val-Ala-Asp-AMC is a photoactive peptide that is activated by light exposure. This drug has cytostatic and cardiotoxic effects, which may be due to its ability to induce autophagy. Z-Val-Ala-Asp-AMC induces cell death in cancer cells through the activation of caspase independent pathways. It also inhibits nitroprusside induced apoptosis in cultured skin cells, suggesting that it may have therapeutic potential for the treatment of cancer.

Formula: C₃₀H₃₄N₄O₉

Purity: Min. 95%

Molecular weight: 594.61 g/mol

Ref: 3D-FV110575

Z-VAD-AMC

CAS: 219137-91-4

Z-VAD-AMC acts as a substrate specifically for caspase-9.

Formula: C₃₀H₃₄N₄O₉

Molecular weight: 594.61

Ref: TM-TP3281