CAS 23491-45-4: Hoechst 33258

Description:Hoechst 33258 is a fluorescent dye commonly used in molecular biology and biochemistry for staining DNA. It is characterized by its ability to bind specifically to the minor groove of double-stranded DNA, which allows for the visualization of nucleic acids under ultraviolet light. The compound exhibits strong fluorescence upon binding, making it a valuable tool for applications such as flow cytometry, fluorescence microscopy, and cell cycle analysis. Hoechst 33258 is typically used in live and fixed cell assays due to its cell-permeable nature. Its chemical structure includes a bisbenzimidazole moiety, which contributes to its DNA-binding properties. The dye is generally considered to have low toxicity, although appropriate safety precautions should be taken when handling it. Additionally, Hoechst 33258 can be used in combination with other fluorescent markers to facilitate multi-parameter analysis in various experimental setups. Overall, its specificity for DNA and fluorescent properties make it an essential reagent in genetic and cellular research.

Formula:C₂₅H₂₄N₆O·3ClH

InChI:InChI=1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI key:InChIKey=SMNPLAKEGAEPJD-UHFFFAOYSA-N

SMILES:Cl.OC=1C=CC(=CC1)C2=NC=3C=CC(=CC3N2)C4=NC5=CC=C(C=C5N4)N6CCN(C)CC6

Synonyms:
2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazinyl)benzimidazole trihydrochloride
2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)-benzimidazole trihydrochloride
2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)benzimidazole trichloride
2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)benzimidazole-3HCl
2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole
4-[5-[5-(4-Methyl-1-piperazinyl)-2-benzimidazolyl]-2-benzimidazolyl]phenol trihydrochloride
4-[6-(4-methylpiperazin-1-yl)-1',3'-dihydro-1H,2'H-2,5'-bibenzimidazol-2'-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride
Bisbenzimide
Bisbenzimide (quenchant)
Bisbenzimide H 33258
See more synonyms
Bisbenzimide trihydrochloride
H 33258
Ho 33258
Hoe 33258
Hoechst 33258
NSC 322921
Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5′-bi-1H-benzimidazol]-2′-yl]-, trihydrochloride
Phenol, 4-[6-(4-methyl-1-piperazinyl)[2,6′-bi-1H-benzimidazol]-2′-yl]-, hydrochloride (1:3)
Phenol, p-[5-[5-(4-methyl-1-piperazinyl)-2-benzimidazolyl]-2-benzimidazolyl]-, trihydrochloride

Found 9 products.

Product data	Purity	Price range	Estimated delivery
Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining] REF: 3B-B6236CAS: 23491-45-4	- - -	51.00 €	Mon 07 Apr 25
Hoechst 33258 REF: IN-DA00I4K8CAS: 23491-45-4	98%	To inquire	Mon 14 Apr 25
Hoechst 33258 trihydrochloride REF: 54-BUP16267CAS: 23491-45-4	96.3%	90.00 €~757.00 €	Tue 15 Apr 25
Bisbenzimide H 33258 [for Biochemical Research] REF: 3B-H1343CAS: 2138819-37-9 23491-45-4	>98.0%(HPLC)	69.00 €	Thu 17 Apr 25
Bis-Benzimide H 33258 REF: 54-BIH1003CAS: 23491-45-4	By hplc: 99.37% (Typical Value in Batch COA)	216.00 €~959.00 €	Mon 21 Apr 25
Hoechst 33258 trihydrochloride REF: TM-T4057CAS: 23491-45-4	95.68% - 96.46%	49.00 €~93.00 €	Mon 21 Apr 25
Bisbenzimide H 33258 Fluorochrome trihydrochloride REF: 7W-GT5082CAS: 23491-45-4	≥ 98.0%	363.00 €~680.00 €	Mon 21 Apr 25
Hoechst 33258 REF: TR-H456600CAS: 23491-45-4	- - -	119.00 €~682.00 €	Thu 24 Apr 25
Hoechst 33258 REF: 3D-FH36311CAS: 23491-45-4	Min. 95%	143.00 €~532.00 €	Tue 27 May 25