CAS 23491-45-4

Hoechst 33258

Description:

Hoechst 33258 is a fluorescent dye commonly used in molecular biology and biochemistry for staining DNA. It is characterized by its ability to bind specifically to the minor groove of double-stranded DNA, which allows for the visualization of nucleic acids under ultraviolet light. The compound exhibits strong fluorescence upon binding, making it a valuable tool for applications such as flow cytometry, fluorescence microscopy, and cell cycle analysis. Hoechst 33258 is typically used in live and fixed cell assays due to its cell-permeable nature. Its chemical structure includes a bisbenzimidazole moiety, which contributes to its DNA-binding properties. The dye is generally considered to have low toxicity, although appropriate safety precautions should be taken when handling it. Additionally, Hoechst 33258 can be used in combination with other fluorescent markers to facilitate multi-parameter analysis in various experimental setups. Overall, its specificity for DNA and fluorescent properties make it an essential reagent in genetic and cellular research.

Formula: C₂₅H₂₄N₆O·3ClH

InChI: InChI=1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H

InChI key: InChIKey=SMNPLAKEGAEPJD-UHFFFAOYSA-N

SMILES: CN1CCN(C=2C=C3NC(=NC3=CC2)C=4C=C5C(=CC4)N=C(N5)C6=CC=C(O)C=C6)CC1.Cl

Synonyms:

• 2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazinyl)benzimidazole trihydrochloride
• 2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)-benzimidazole trihydrochloride
• 2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)benzimidazole trichloride
• 2-[2-(4-Hydroxyphenyl)-6-benzimidazolyl]-6-(1-methyl-4-piperazyl)benzimidazole-3HCl
• 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole
• 4-[5-[5-(4-Methyl-1-piperazinyl)-2-benzimidazolyl]-2-benzimidazolyl]phenol trihydrochloride
• 4-[6-(4-methylpiperazin-1-yl)-1',3'-dihydro-1H,2'H-2,5'-bibenzimidazol-2'-ylidene]cyclohexa-2,5-dien-1-one trihydrochloride
• Bisbenzimide
• Bisbenzimide (quenchant)
• Bisbenzimide H 33258
• Bisbenzimide trihydrochloride
• H 33258
• Ho 33258
• Hoe 33258
• Hoechst 33258
• NSC 322921
• Phenol, 4-[5-(4-methyl-1-piperazinyl)[2,5′-bi-1H-benzimidazol]-2′-yl]-, trihydrochloride
• Phenol, 4-[6-(4-methyl-1-piperazinyl)[2,6′-bi-1H-benzimidazol]-2′-yl]-, hydrochloride (1:3)

Bisbenzimide H 33258 [for Biochemical Research]

CAS: 2138819-37-9, 23491-45-4

Formula: C₂₅H₂₄N₆O·3HCl

Purity: >98.0%(HPLC)

Color and Shape: Light yellow to Brown to Dark green powder to crystal

Molecular weight: 533.88

Ref: 3B-H1343

Bisbenzimide H 33258 (0.2mL×5) (1mg/mL in Water) [for Cell Staining]

CAS: 23491-45-4

Formula: C₂₅H₂₄N₆O·3HCl

Color and Shape: Light yellow to Yellow clear liquid

Molecular weight: 533.88

Ref: 3B-B6236

Hoechst 33258

CAS: 23491-45-4

Formula: C₂₅H₂₇Cl₃N₆O

Purity: 98%

Color and Shape: Solid

Molecular weight: 533.8805

Ref: IN-DA00I4K8

Hoechst 33258 trihydrochloride

CAS: 23491-45-4

Hoechst 33258 trihydrochloride

Purity: ≥95%

Molecular weight: 533.9g/mol

Ref: 54-BUP16267

Bis-Benzimide H 33258

CAS: 23491-45-4

Bis-Benzimide H 33258

Formula: C₂₅H₂₄N₆O·3ClH

Purity: By hplc: 99.37% (Typical Value in Batch COA)

Color and Shape: yellow solid

Molecular weight: 533.88g/mol

Ref: 54-BIH1003

Hoechst 33258 trihydrochloride

CAS: 23491-45-4

Hoechst 33258 trihydrochloride (Bisbenzimide) is a benzimidazole anti-filarial agent. Hoechst 33258 is fluorescent when it binds to certain nucleotides in DNA.

Formula: C₂₅H₂₄N₆O·3HCl

Purity: 95.68% - 99.47%

Color and Shape: Yellow-Green Powder

Molecular weight: 533.9

Ref: TM-T4057

Bisbenzimide H 33258 Fluorochrome trihydrochloride

CAS: 23491-45-4

Formula: C₂₅H₂₄N₆O·3HCl

Purity: ≥ 98.0%

Color and Shape: Yellow to dark yellow powder

Molecular weight: 533.9

Ref: 7W-GT5082

Hoechst 33258

CAS: 23491-45-4

Formula: C₂₅H₂₄N₆O·3ClH

Color and Shape: Neat

Molecular weight: 533.88

Ref: TR-H456600

Hoechst 33258

CAS: 23491-45-4

Hoechst 33258 is a fluorescent dye that binds to the DNA of cells and can be used as a model system in cell biology. It is also used to detect radiation exposure and has been shown to inhibit the growth of certain bacteria. Hoechst 33258 binds with high affinity to basic proteins, such as histones, which are rich in arginine. This interaction inhibits the ability of DNA polymerase to synthesize DNA and prevents cellular transformation. The drug also inhibits cellular physiology by inhibiting protein synthesis, which may lead to cell death. Hoechst 33258 is not active against most bacteria because it has low affinity for phosphate groups on the bacterial cell wall.

Formula: C₂₅H₂₄N₆O•(HCl)₃

Purity: Min. 95%

Color and Shape: Yellow Powder

Molecular weight: 533.88 g/mol

Ref: 3D-FH36311