BP Fluor 488 Hydrazide is a fluorescent dye commonly used in biological and chemical research, particularly for labeling and imaging applications. It is characterized by its bright green fluorescence, which is excited by light in the blue to ultraviolet range. The hydrazide functional group allows for specific conjugation to various biomolecules, such as proteins and nucleic acids, facilitating the study of cellular processes and interactions. This compound is typically soluble in polar solvents, making it suitable for use in aqueous biological systems. Its stability under physiological conditions and compatibility with various labeling techniques enhance its utility in fluorescence microscopy and flow cytometry. Additionally, BP Fluor 488 Hydrazide is often employed in assays that require high sensitivity and specificity, contributing to advancements in fields such as molecular biology, biochemistry, and medical diagnostics. Safety precautions should be observed when handling this compound, as with any chemical reagent, to minimize exposure and ensure proper laboratory practices.

This product is a hydrazide derivative of AF 488 dye. Hydrazides efficiently react with aldehydes and ketones, resulting in hydrazones, so that this compound can be used for conjugation with carbonyl derivatives of biomolecules.
The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with hydrazides). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can also be conjugated with hydrazides in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.
AF 488 is a bright and photostable dye. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.
AF 488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated, otherwise, the use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF 488.

BP Fluor 488 Hydrazide

2500973-94-2: BP Fluor 488 Hydrazide is a fluorescent dye commonly used in biological and chemical research, particularly for labeling and imaging applications. It is characterized by its bright green fluorescence, which is excited by light in the blue to ultraviolet range. The hydrazide functional group allows for specific conjugation to various biomolecules, such as proteins and nucleic acids, facilitating the study of cellular processes and interactions. This compound is typically soluble in polar solvents, making it suitable for use in aqueous biological systems. Its stability under physiological conditions and compatibility with various labeling techniques enhance its utility in fluorescence microscopy and flow cytometry. Additionally, BP Fluor 488 Hydrazide is often employed in assays that require high sensitivity and specificity, contributing to advancements in fields such as molecular biology, biochemistry, and medical diagnostics. Safety precautions should be observed when handling this compound, as with any chemical reagent, to minimize exposure and ensure proper laboratory practices.

<p>Green fluorescent dye used for labelling aldehydes or ketones in polysaccharides or glycoproteins with an excitation peak at 499 nm and an emission peak at 520 nm. Frequently employed in microscopy and flow cytometry.</p>

CAS 2500973-94-2

BP Fluor 488 Hydrazide

BP Fluor 488 hydrazide

Ref: 3D-FB184557