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CAS 25405-62-3

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O-nitrophenyl B-D-galactopyranoside*6-phosphate

Description:
O-nitrophenyl β-D-galactopyranoside 6-phosphate, commonly referred to as ONPG-6-P, is a synthetic compound used primarily in biochemical research, particularly in enzyme assays. It is a derivative of galactose and serves as a substrate for β-galactosidase, an enzyme that catalyzes the hydrolysis of glycosidic bonds in carbohydrates. The presence of the nitrophenyl group allows for colorimetric detection, as the hydrolysis of ONPG-6-P releases o-nitrophenol, which can be quantified spectrophotometrically. This compound is typically soluble in water and exhibits stability under standard laboratory conditions. Its phosphate group enhances its polarity, influencing its interaction with biological systems. ONPG-6-P is valuable in studying enzyme kinetics and carbohydrate metabolism, providing insights into various biological processes. As with many chemical substances, proper handling and safety precautions are essential, as it may pose risks if ingested or improperly managed.
Formula:C24H36NO11P
InChI:InChI=1/C24H36NO11P/c26-21-20(34-24(23(28)22(21)27)33-19-14-8-7-13-18(19)25(29)30)15-32-37(31,35-16-9-3-1-4-10-16)36-17-11-5-2-6-12-17/h7-8,13-14,16-17,20-24,26-28H,1-6,9-12,15H2/t20-,21+,22+,23-,24-/m1/s1
Synonyms:
  • dicyclohexyl [(2R,3R,4S,5R,6S)-3,4,5-trihydroxy-6-(2-nitrophenoxy)tetrahydropyran-2-yl]methyl phosphate
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