CAS 254109-22-3

4-Amino-5-methylamino-2′,7′-difluorofluorescein diacetate

Description:

4-Amino-5-methylamino-2′,7′-difluorofluorescein diacetate, with CAS number 254109-22-3, is a fluorescent dye commonly used in biological and chemical research. This compound is characterized by its ability to emit fluorescence upon excitation, making it valuable for applications such as cell imaging and flow cytometry. The presence of amino groups enhances its solubility in aqueous environments, while the diacetate groups facilitate cellular uptake, allowing for effective labeling of live cells. The difluorofluorescein structure contributes to its strong fluorescence properties, with specific excitation and emission wavelengths that can be utilized in various assays. Additionally, this compound is often employed in studies involving reactive oxygen species and other cellular processes, providing insights into biological mechanisms. Its stability and photophysical properties make it a versatile tool in the field of biochemistry and molecular biology. However, as with any chemical substance, proper handling and safety precautions should be observed to mitigate any potential hazards associated with its use.

Formula: C₂₅H₁₈F₂N₂O₇

InChI: InChI=1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChI key: InChIKey=BEVHTVRRVVEMEF-UHFFFAOYSA-N

SMILES: O=C1OC2(C=3C(OC=4C2=CC(F)=C(OC(C)=O)C4)=CC(OC(C)=O)=C(F)C3)C=5C1=C(N)C(NC)=CC5

Synonyms:

• 3′,6′-Bis(acetyloxy)-4-amino-2′,7′-difluoro-5-(methylamino)spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one
• 4-Amino-5-methylamino-2′,7′-difluorofluorescein diacetate
• Daf-Fm Da
• Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 3′,6′-bis(acetyloxy)-4-amino-2′,7′-difluoro-5-(methylamino)-

4-Amino-5-methylamino-2',7'-difluorofluoresceindiacetate

CAS: 254109-22-3

Formula: C₂₅H₁₈F₂N₂O₇

Purity: 98%

Color and Shape: Solid

Molecular weight: 496.4164

Ref: IN-DA00BF5V

DAF-FM DA

CAS: 254109-22-3

DAF-FM DA

Purity: 98%

Ref: 54-BI12246

DAF-FM DA

CAS: 254109-22-3

DAF-FM DA detects and measures low NO levels via fluorescein-compatible fluorescence.

Formula: C₂₅H₁₈F₂N₂O₇

Purity: 98%

Color and Shape: Solid

Molecular weight: 496.42

Ref: TM-T18965

DAF-FM DA (cell permeable)

Controlled Product

CAS: 254109-22-3

Applications DAF-FM DA (cell permeable) is a cell-permeable analog of DAF-FM that acts as a cell permeable NO indicator or detection.
References Mainz, E. R., et al.: Anal. Methods, 4, 414-420 (2012); Xian, J., et al.: Fish Shellfish Immunol., 35, 2032-2039 (2013)

Formula: C₂₅H₁₈F₂N₂O₇

Color and Shape: Neat

Molecular weight: 496.416

Ref: TR-D193325