CAS 298-83-9

Nitro Blue Tetrazolium

Description:

Nitro Blue Tetrazolium (NBT) is a chemical compound commonly used as a redox indicator and in various biochemical assays. It is characterized by its ability to undergo reduction, resulting in a color change that can be visually monitored, making it useful in detecting superoxide radicals and assessing cellular metabolic activity. NBT is a yellow crystalline powder that is soluble in water and exhibits a distinctive blue color upon reduction. Its chemical structure includes a tetrazolium ring, which is crucial for its redox properties. In biological research, NBT is often employed in histochemical staining to visualize oxidative stress and in assays to measure the activity of enzymes such as dehydrogenases. Additionally, it is utilized in the study of various cellular processes, including apoptosis and inflammation. Safety precautions should be taken when handling NBT, as it can be harmful if ingested or inhaled, and appropriate protective equipment should be used.

Formula: C₄₀H₃₀Cl₂N₁₀O₆

InChI: InChI=1/C40H32N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3,(H,41,43)(H,42,44);2*1H

InChI key: InChIKey=FSVCQIDHPKZJSO-UHFFFAOYSA-L

SMILES: O(C)C1=C(N2[N+](=NC(=N2)C3=CC=CC=C3)C4=CC=C(N(=O)=O)C=C4)C=CC(=C1)C5=CC(OC)=C(C=C5)N6[N+](=NC(=N6)C7=CC=CC=C7)C8=CC=C(N(=O)=O)C=C8.[Cl-]

Synonyms:

• 2,2'-Bis(p-nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-biphenylylene)ditetrazolium chloride
• 2,2'-Bis(p-nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride
• 2,2'-Di-p-nitrophenyl-5,5'-diphenyl-3,3'-bis(3,3'-dimethoxy-4,4'-biphenylene)ditetrazolium chloride
• 2,2'-Dinitrophenyl-5,5'-diphenyl-3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride
• 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, chloride (1:2)
• 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy-4,4'-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl-, dichloride
• 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-, dichloride
• 3,3'-(3,3'-Dimethoxy-4,4'-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]
• 3,3'-(3,3'-Dimethoxy-4,4'-diphenylene)bis[2-(p-nitrophenyl)-5-phenyltetrazolium chloride]
• 3,3'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium dichloride
• 5,5'-Diphenyl-3,3'-Bis(4-Nitrophenyl)-2,2'-(3,3'-Dimethoxybiphenyl-4,4'-Ylene)Ditetrazolium Dichloride
• 5,5'-Diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylen)ditetrazoliumdichlorid
• Dichlorure De 5,5'-Diphenyl-3,3'-Bis(4-Nitrophenyl)-2,2'-(3,3'-Dimethoxybiphenyle-4,4'-Ylene)Ditetrazolium
• Dichlorure de 5,5'-diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylene)ditetrazolium
• Dicloruro De 5,5'-Difenil-3,3'-Bis(4-Nitrofenil)-2,2'-(3,3'-Dimetoxibifenil-4,4'-Ilen)Ditetrazolio
• NBT
• NBT (dye)
• NTB
• Nitro BT
• Nitro Blue Tetrazolium
• Nitro Blue Tetrazolium chloride
• Nitro TB
• Nitro Tetrazolium BT
• Nitrotetrazolium Chloride Blue
• Nitrotetrazolium blue
• Nsc 27622
• Tetrazolium Nitro BT
• Tetrazolium nitro blue
• Tetrazolium, 3,3'-(3,3'-dimethoxy-4,4'-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl-2H-chloride]
• p-NBT
• p-Nitro blue tetrazolium
• p-Nitro blue tetrazolium chloride
• p-Nitrotetrazolium blue
• Nitrotetragolium blue chloride
• 3,3'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium]
• nitrotetrazoliumbt
• nitrotetrazoliumchlorideblue
• Nitrotetrazolium Blue chloride monohydrate
• 2H-Tetrazolium, 2,2′-(3,3′-dimethoxy[1,1′-biphenyl]-4,4′-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, dichloride
• 2h-tetrazolium,3,3'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(2-(4-nitrophe
• Nitrotetrazoliumblau
• tetrazoliumnitroblue
• nitrobluetetrazoliumsalt
• 2h-tetrazolium,3,3'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[2-(4-nitrophe
• 2,2'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-2,3-dihydro-1H-tetrazol-3-ium] dichloride
• tetrazoliumnitrobt
• 3,3'-(3,3'-dimethoxybiphenyl-4,4'-diyl)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium] dichloride
• Nitrotetrazolium Blue chloride
• Nitrotetrazolium Blu
• p-Nitro-Blue tetrazolium chlo
• Nitro blue tetrazolium >99%
• NITRO BLUE TETRAZOLIUM 10 MG TABLETS,*TR U-MEASURE C
• 2,2'-DI-P-NITROPHENYL-5,5'DIPHENYL-[3,3'-DIMETHOXY-4,4'-DIPHENYLENE] DITETRAZOLIUM CHLORIDE
• 3,3'-[3,3-Dimethoxy-(1,1-biphenyl)-4,4-diyl]-bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchloride]
• Nitro BT~Nitrotetrazolium Blue chloride monohydrate
• Nitro Blue tetrazolium chloride (NBT)
• NITRO BLUE TETRAZOLIUM CHLORIDE (NBT) FO
• p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT, 3,3μ-(3,3μ-Dimethoxy-4,4μ-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,2μ-bis(4-Nitrophenyl)-5,5μ-diphenyl-3,3μ-(3,3μ-dimethoxy-4,4μ-diphenylene)ditetrazolium chloride
• [1,10-biphenyl]-4,40-diyl)bis[3-(4-nitrophenyl)-5-phenyl]-,
• nitrotetrazolium Blue Nitro Blue Tetrazolium (nbt)
• Nitro Blue Tetrazolium Chloride monohydrate,85%
• NBT Vials
• Nbt(NitroblueTetrazoliumChloride)
• 5,50-diphenyl-3,30-(3,30-dimethoxy-4,40-diphenylene)
• NITRO BLUE TETRAZOLIUM CHLORIDE MONOHYDRATE: TECH., 90%
• 20-bis(p-nitrophenyl)-5,50-diphenyl-3,30-(3,30-dimethoxy-
• BUFFER PHOSPHATE BUTTERFIELD (4X10)
• NITRO BLUE TETRAZOLIUM MOLECULAR*BIOLOGY REAGENT
• 2,2'-Di-p-nitrophenyl-5,5'-diphenyl-(3,3'-dimethoxy)-4,4'-bisphenyleneditetrazolium Chloride
• 2,2'-DI-P-NITROPHENYL-5,5'-DIPHENYL-(3,3'-DIMETHOXY)-4,4'-BISPHENYLENEDITETRAZOLIUM CHLORIDE
• Nsc
• NBT CHLORIDE
• NITRO TETRAZOLIUM BLUE
• 2,2'-Bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride
• 2-(3,3'-Dimethoxy-4'-(2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium-3-yl)-[1,1'-biphenyl]-4-yl)
• 4,40-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl]-,
• 2,
• 2h-tetrazolium, 2,20-(3,30-dimethoxy
• p-Nitro-Blue tetra
• NITRO BLUE TETRAZOLIUMCHLORIDE ANALYTICAL GRADE
• 2,20-di-p-nitrophenyl-
• 4-NITRO BLUE TETRAZOLIUM CHLORIDE
• NITRO BLUE TETRAZOLIUM CHLORIDE MONOHYDRATE extrapure AR
• 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)-BIS-(2-P-NITROPHENYL)-5-(PHENYL)-2H-TETRAZOLIUM CHLORIDE
• P-NITROBLUE TETRAZOLIUM DICHLORIDE
• p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT, Nitrotetrazolium Blue chloride, 3,3μ-(3,3μ-Dimethoxy-4,4μ-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], 2,2μ-bis(4-Nitrophenyl)-5,5μ-diphenyl-3,3μ-(3,3μ-dimethoxy-4,4μ-diphenylene)ditetrazolium chloride
• 2,2'-DI-4-NITROPHENYL-5,5'-DIPHENYL-3,3'-(3,3'-DIMETHOXY-4,4'-DIPHENYLENE)-DITETRAZOLIUM-CHLORIDE
• NITRO BLUE TETRAZOLIUM (NBT) REDUCTION
• 27622
• phenyltetrazolium Chloride]
• Nitrotetrazolium Blue chloride,2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-
• dichloride
• NITROTETRAZOLIUM BLUE CHLOR. PGE W. 10
• Nitro Blue Tetrazolium Chloride ,98%
• Nitro Blue Tetrazolium chloride, tech. 90%
• bis[2-(p-nitrophenyl)-5-phenyl-2h-chloride]
• 3,30-dimethoxy-4,40-diphenylene)ditetrazolium Chloride
• TETRAZOLIUM, (3,3'-DIMETHOXY-4,4'-BIPHENYLENE)BIS[2-(4-NITROPHENYL)-5-PHENYL-2H]-, CHLORIDE
• 3,30-(3,30-dimethoxy-4,40-biphenylylene)bis[2-(p-nitrophenyl)-
• 3,30-(3,30-
• Nitro Blue Tetrazolium chloride, technical, 90%
• NBT (Nitrobluetetrazolium chloride blue)
• Nitro Blue Tetrazolium Chloride monohydrate,90%
• NitroBlueTetrazoliumA.R.
• 2h-tetrazolium, 3,30-(3,30-dimethoxy-
• DITETRAZOLIUM DYE
• NitroterazoiuM blue chlorid (NBT)
• Tetrazolium, 3,30-(3,30-dimethoxy-4,40-biphenylylene)
• P-NITROTETRAZOLIUM BLUE CHLORIDE
• 2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-
• Nitro Blue Tetrazolium [for Biochemical Research]
• P-NitroBlueTetrazolliumChloride
• Nitro Blue tetrazolium chloride p.a.
• P-
• 5-phenyl-2h-tetrazolium Chloride]
• 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)BIS[2-(P-NITROPHENYL)-5-PHENYLTETRAZOLIUM] CHLORIDE
• 2,2'-BIS(4-NITROPHENYL)-5,5'-DIPHENYL-3,3'-(3,3'-DIMETHOXY-4,4'-DIPHENYLENE)DITETRAZOLIUM CHLORIDE
• NITRO BLUE TETRAZOLIUM GRADE III*CRYSTAL LINE
• P-NITROBLUETETRAZOLIUMCHLORIDE,CRYSTAL,REAGENT
• NITROBLUETETRAZOLIN
• p-Nitro-Blue tetrazolium chlori
• Nitrotetrazolium Blue chloride ,90%
• 5,50-diphenyl-3,30-bis(3,30-dimethoxy-4,40-biphenylene)
• NITROBLUE TETRAZOLIUM CHLORIDE BIOTECH GRADE
• 3,3'-(3,3'-DIMETHOXY-4,4'-BIPHENYLENE)BIS[2-(4-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM CHLORIDE]
• 4,40-biphenylylene)ditetrazolium Chloride
• nitro blue tetrazolium dichloride
• NBTMF
• SOAP SOLUTION STABLE
• Nitrotetrazolium blue chloride(NBT)
• 2,20-dinitrophenyl-5,50-diphenyl-
• 2,20-bis(p-nitrophenyl)-
• Nitro BT (Nitro blue tetrazolium chloride)
• 3,3'-(3,3'-Dimethoxy-[1,1'-biphenyl]-4,4'-diyl)bis(2-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium) chloride
• dimethoxy-4,40-diphenylene)bis[2-(p-nitrophenyl)-5-
• 2H-Tetrazolium, 2,2-(3,3-dimethoxy1,1-biphenyl-4,4-diyl)bis3-(4-nitrophenyl)-5-phenyl-, dichloride

Nitro Blue Tetrazolium [for Biochemical Research]

CAS: 298-83-9

Formula: C₄₀H₃₀Cl₂N₁₀O₆

Purity: >98.0%(T)(HPLC)

Color and Shape: Light yellow to Brown powder to crystal

Molecular weight: 817.64

Ref: 3B-D0844

Nitro Blue Tetrazolium chloride, tech. 90%

CAS: 298-83-9

Nitro Blue Tetrazolium chloride is used to confirm diagnosis of chronic granulomatous disease. It is a microscopic stain, which is used to detect dehydrogenases and alkaline phosphatase. It acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Further, it is used i

Formula: C₄₀H₃₀Cl₂N₁₀O₆

Purity: 90%

Color and Shape: Yellow, Powder

Molecular weight: 817.64

Ref: 02-B23792

Nitro Blue Tetrazolium chloride, 98+%

CAS: 298-83-9

Intense blue dye used in histochemistry determinations

Formula: C₄₀H₃₀Cl₂N₁₀O₆

Purity: 98+%

Color and Shape: Powder, Yellow

Molecular weight: 817.64

Ref: 02-J60230

2H-Tetrazolium, 2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-, chloride (1:2)

CAS: 298-83-9

Formula: C₄₀H₃₀Cl₂N₁₀O₆

Purity: 95%

Color and Shape: Solid

Molecular weight: 817.6356

Ref: IN-DA0039OO

Nitro blue tetrazolium

CAS: 298-83-9

Nitro blue tetrazolium

Formula: C₄₀H₃₀N₁₀O₆·2Cl

Purity: Purity: >98% solubility in (Typical Value in Batch COA)

Color and Shape: light yellow/green powder

Molecular weight: 817.6356g/mol

Ref: 54-BIMB1019

Nitroblue tetrazolium chloride

CAS: 298-83-9

Formula: C₄₀H₃₀Cl₂N₁₀O₆

Purity: (HPLC) ≥ 95.0%

Color and Shape: Faint yellow to yellow powder

Molecular weight: 817.65

Ref: 7W-GC4263

Nitroblue tetrazolium chloride, 98.0%

CAS: 298-83-9

Formula: C₄₀H₃₀Cl₂N₁₀O₆

Purity: (HPLC) ≥ 98.0%

Color and Shape: Faint yellow to yellow powder

Molecular weight: 817.65

Ref: 7W-GE5978

Nitro blue tetrazolium chloride

CAS: 298-83-9

Nitro blue tetrazolium chloride

Purity: ≥98%

Molecular weight: 817.64g/mol

Ref: 54-BUP18833

Nitro blue tetrazolium chloride

CAS: 298-83-9

NBT: inhibits NO synthase (IC50=3-4M), scavenges superoxide; used with BCIP in blotting/staining.

Formula: C₄₀H₃₀Cl₂N₁₀O₆

Purity: 98.02% - 99.08%

Color and Shape: Yellow Crystals

Molecular weight: 817.64

Ref: TM-T19032

Nitrotetrazolium Blue Chloride (Technical Grade)

Controlled Product

CAS: 298-83-9

Applications Nitrotetrazolium Blue Chloride is a Tetrazolium dye used in colorimetric assays. Dyes and metabolites.
References Berridge, Michael V., et al.: Biotechnol. Annu. Rev., 11, 127 (2005)

Formula: C₄₀H₃₀N₁₀O₆•2(Cl)

Color and Shape: Neat

Molecular weight: 746.7323545

Ref: TR-N572335

Nitroblue tetrazolium chloride

CAS: 298-83-9

Nitroblue tetrazolium chloride is colorless to yellow dye that is reducible to blue or black formazan crystals by certain cells; formerly used to distinguish between nonbacterial and bacterial diseases, the latter causing neutrophils to reduce the dye; used to confirm diagnosis of chronic granulomatous disease.

Formula: C₄₀H₃₀Cl₂N₁₀O₆

Purity: Min. 98 Area-%

Color and Shape: White Yellow Powder

Molecular weight: 817.64 g/mol

Ref: 3D-EN13587

Nitrotetrazolium blue chloride

CAS: 298-83-9

NBT is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) (B-7450, B-7500, B-7505) to detect alkalinephosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purplish-brown final color of the precipitates at the reactionsites. NBT reduction Test: Assessment of reduction of NBT dye by stimulated and unstimulated neutrophils.

Formula: C₄₀H₃₀Cl₂N₁₀O₆

Molecular weight: 817.65 g/mol

Ref: 3D-N-8100

Nitro Blue Tetrazolium Chloride (Nitro BT) (NBT) extrapure AR, 99%

CAS: 298-83-9

Formula: C₄₀H₃₀N₁₀O₆Cl₂

Purity: min. 99%

Color and Shape: Lemon yellow, Crystalline powder, Clear, Yellow

Molecular weight: 817.65

Ref: SR-11207

Nitro Blue Tetrazolium Chloride (Nitro BT) (NBT) for tissue culture, 99%

CAS: 298-83-9

Formula: C₄₀H₃₀N₁₀O₆Cl₂

Purity: min. 99%

Color and Shape: Lemon yellow, Crystalline powder, Clear, Yellow

Molecular weight: 817.65

Ref: SR-13001

Nitro Blue Tetrazolium Chloride (Nitro BT) (NBT) for molecular biology, 99%

CAS: 298-83-9

Formula: C₄₀H₃₀N₁₀O₆Cl₂

Purity: min. 99%

Color and Shape: Lemon yellow, Crystalline powder, Clear, Yellow

Molecular weight: 817.65

Ref: SR-48898

Nitro blue tetrazolium

CAS: 298-83-9

M02729 - Nitro blue tetrazolium

Formula: C₄₀H₃₀Cl₂N₁₀O₆

Purity: >98.0%(HPLC)(T)

Color and Shape: Solid, Pale yellow to reddish yellow powder

Molecular weight: 817.64

Ref: 10-M02729