CAS 3012-71-3
:fluorescein isothiocyanate isomer ii
Description:
Fluorescein isothiocyanate isomer II (CAS 3012-71-3) is a fluorescent dye commonly used in biological and biochemical applications, particularly in labeling proteins and antibodies for fluorescence microscopy and flow cytometry. This compound is characterized by its bright green fluorescence, which is attributed to its fluorescein backbone. It features an isothiocyanate functional group, which allows for covalent bonding to amino groups in proteins, facilitating the conjugation process. The dye is soluble in organic solvents and water, making it versatile for various experimental conditions. Its fluorescence properties can be influenced by pH and the environment, which is crucial for applications in live-cell imaging. Additionally, fluorescein isothiocyanate isomer II is known for its stability under standard laboratory conditions, although it should be protected from light to prevent photobleaching. Safety precautions should be taken when handling this compound, as it may cause irritation upon contact with skin or eyes. Overall, its unique properties make it a valuable tool in molecular biology and biochemistry research.
Formula:C21H11NO5S
InChI:InChI=1/C21H11NO5S/c23-12-2-5-15-18(8-12)26-19-9-13(24)3-6-16(19)21(15)17-7-11(22-10-28)1-4-14(17)20(25)27-21/h1-9,23-24H
SMILES:c1cc2c(cc1N=C=S)C1(c3ccc(cc3Oc3cc(ccc13)O)O)OC2=O
Synonyms:- Fluorescein-6-isothiocyanate
- FITC Isomer II
- 3',6'-dihydroxy-6-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
- 3',6'-Dihydroxy-6-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
- Fluorescein-4-isothiocyanate
- Fitc-Ii
- 6-Fluorescein Isothiocyanate
- 6-Fitc
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