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CAS 3150-25-2

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3-Nitrophenyl β-D-galactopyranoside

Description:
3-Nitrophenyl β-D-galactopyranoside, with the CAS number 3150-25-2, is a synthetic compound commonly used as a substrate in biochemical assays, particularly for the detection of β-galactosidase activity. This compound features a galactopyranoside moiety linked to a 3-nitrophenyl group, which imparts specific chemical properties. It is typically a white to pale yellow crystalline solid that is soluble in water and organic solvents, making it versatile for laboratory applications. Upon hydrolysis by β-galactosidase, it releases 3-nitrophenol, which can be quantified spectrophotometrically, allowing for the measurement of enzyme activity. The presence of the nitro group enhances the compound's reactivity and provides a chromogenic response, which is crucial for visualizing enzymatic reactions. Additionally, 3-Nitrophenyl β-D-galactopyranoside is stable under standard laboratory conditions but should be stored properly to maintain its integrity. Its use in research and diagnostics underscores its importance in enzymology and molecular biology.
Formula:C12H15NO8
InChI:InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-3-1-2-6(4-7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
InChI key:InChIKey=VCCMGHVCRFMITI-YBXAARCKSA-N
SMILES:O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1)C2=CC(N(=O)=O)=CC=C2
Synonyms:
  • 3-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactopyranoside
  • 3-nitrophenyl beta-D-galactopyranoside
  • Galactopyranoside, m-nitrophenyl
  • Galactopyranoside, m-nitrophenyl, β-<span class="text-smallcaps">D</span>-
  • m-Nitrophenyl beta-D-galactopyranoside
  • m-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactopyranoside
  • m-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactoside
  • m-Nitrophenyl β-galactoside
  • β-<span class="text-smallcaps">D</span>-Galactopyranoside, 3-nitrophenyl
  • Galactopyranoside, m-nitrophenyl, β-D-
  • See more synonyms
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