CAS 3301-79-9

6-Carboxyfluorescein

Description:

6-Carboxyfluorescein is a fluorescent dye commonly used in biochemical and molecular biology applications. It is characterized by its bright green fluorescence, which is attributed to its xanthene structure. The compound has a carboxylic acid functional group, which enhances its solubility in aqueous solutions, making it suitable for various biological assays. Its fluorescence properties make it an excellent choice for labeling biomolecules, tracking cellular processes, and as a tracer in flow cytometry. The dye exhibits a high quantum yield and is relatively stable under physiological conditions, although it can be sensitive to pH changes and light exposure. Additionally, 6-Carboxyfluorescein can be conjugated to proteins or nucleic acids, allowing for versatile applications in imaging and detection. Its ability to undergo resonance energy transfer also makes it useful in fluorescence resonance energy transfer (FRET) studies. Overall, 6-Carboxyfluorescein is a valuable tool in research for visualizing and quantifying biological interactions and processes.

Formula: C₂₁H₁₂O₇

InChI: InChI=1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key: InChIKey=BZTDTCNHAFUJOG-UHFFFAOYSA-N

SMILES: O=C1OC2(C=3C1=CC=C(C(O)=O)C3)C=4C(OC=5C2=CC=C(O)C5)=CC(O)=CC4

Synonyms:

• 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-6-carboxylic acid
• 3′,6′-Dihydroxy-1-oxospiro[2-benzofuran-3,9′-xanthene]-5-carboxylic acid
• 3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-6-carboxylic acid
• 6-Carboxy Fluorescein
• 6-Fam
• Acide 3',6'-dihydroxy-3-oxospiro[isobenzofurane-1(3H),9'-xanthene]-6-carboxylique
• Fluorescein, 6-carboxy-
• Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid, 3',6'-dihydroxy-3-oxo-
• Spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-6-carboxylic acid, 3′,6′-dihydroxy-3-oxo-
• Spiro[phthalan-1,9'-xanthene]-6-carboxylic acid, 3',6'-dihydroxy-3-oxo-
• Spiro[phthalan-1,9′-xanthene]-6-carboxylic acid, 3′,6′-dihydroxy-3-oxo-

6-Carboxyfluorescein

CAS: 3301-79-9

Formula: C₂₁H₁₂O₇

Purity: >97.0%(HPLC)

Color and Shape: Light yellow to Brown powder to crystal

Molecular weight: 376.32

Ref: 3B-C2478

6-Carboxy-fluorescein

CAS: 3301-79-9

Widely used green-fluorescent labeling dye.

Formula: C₂₁H₁₂O₇

Purity: 99.1%

Color and Shape: Orange Powder

Molecular weight: 376.32

Ref: 01-4045142

6-Carboxyfluorescein

CAS: 3301-79-9

Formula: C₂₁H₁₂O₇

Purity: 95%

Color and Shape: Solid

Molecular weight: 376.3158

Ref: IN-DA0038TE

6-FAM

CAS: 3301-79-9

6-FAM

Purity: ≥95%

Molecular weight: 376.32g/mol

Ref: 54-BUP11738

6-Carboxyfluorescein

CAS: 3301-79-9

6-Carboxyfluorescein

Purity: 97%

Molecular weight: 376.32g/mol

Ref: 54-BIC1051

6-Carboxyfluorescein

CAS: 3301-79-9

Formula: C₂₁H₁₂O₇

Purity: ≥ 97.0%

Color and Shape: Yellow to orange-red powder

Molecular weight: 376.32

Ref: 7W-GT6690

6-FAM

CAS: 3301-79-9

6-Carboxyfluorescein (6-FAM) contains a carboxylic acid functional group, facilitating reactions with primary amines through carbodiimide activation.

Formula: C₂₁H₁₂O₇

Purity: 97.23% - 98.73%

Color and Shape: Orange Solid

Molecular weight: 376.32

Ref: TM-T18902

6-Carboxyfluorescein

Controlled Product

CAS: 3301-79-9

Applications 6-Carboxyfluorescein is a useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derived reagents. Dyes and metabolites.
References Theisen, P., et al.: Tetrahedron Lett., 33, 5033 (1992); Mattingly, P.: Bioconjgate Chem., 3, 430 (1992); Bertrand, R., et al.: Biochemistry, 34, 9500 (1995);

Formula: C₂₁H₁₂O₇

Color and Shape: Dark Orange

Molecular weight: 376.32

Ref: TR-C178325

6-Carboxyfluorescein

CAS: 3301-79-9

6-Carboxyfluorescein is a fluorescent dye widely used in PCR methodologies. It is used to label the 5’ end of oligonucleotides as a reporter in the presence of a quencher at the 3’ end (dual labelled probe). During amplification, the dye is cleaved, and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. It is used to tag DNA and RNA for in vivo and in vitro applications, as well as for structural studies and RNA-protein interactions. The carboxylic acid can be activated to react with a primary amine. It is a 5’-reporter used in RT-PCR for COVID-19 diagnosis by HKI; Institute Pasteur; Charité; CDC, USA; CDC, China; NIID and NIH, Thailand. (λex/λem: 495/517nm in DMSO or DMF at pH > 9.0).

Formula: C₂₁H₁₂O₇

Purity: Min. 96 Area-%

Color and Shape: Red Powder

Molecular weight: 376.32 g/mol

Ref: 3D-FC19774

6-Carboxyfluorescein

CAS: 3301-79-9

Formula: C₂₁H₁₂O₇

Purity: 95%

Color and Shape: Solid, Pale yellow to yellow red powder

Molecular weight: 376.32

Ref: 10-F214836