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CAS 3348-03-6

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6-carboxyfluorescein diacetate

Description:
6-Carboxyfluorescein diacetate (CAS 3348-03-6) is a fluorescent dye commonly used in biological and biochemical applications, particularly for cell viability assays and as a tracer in various experimental setups. This compound is a non-fluorescent, membrane-permeable ester that, upon entering live cells, is hydrolyzed by intracellular esterases to yield the fluorescent 6-carboxyfluorescein. The resulting compound exhibits strong fluorescence, making it useful for detecting live cells and assessing cellular functions. It has a characteristic absorption and emission spectrum, typically in the green region, which allows for easy visualization using standard fluorescence microscopy techniques. Additionally, 6-carboxyfluorescein diacetate is relatively stable under physiological conditions, although it can be sensitive to light and should be stored in a dark environment to prevent degradation. Its ability to indicate cell membrane integrity and metabolic activity makes it a valuable tool in various fields, including cell biology, pharmacology, and toxicology.
Formula:C25H16O9
InChI:InChI=1/C25H16O9/c1-12(26)31-15-4-7-18-21(10-15)33-22-11-16(32-13(2)27)5-8-19(22)25(18)20-9-14(23(28)29)3-6-17(20)24(30)34-25/h3-11H,1-2H3,(H,28,29)
SMILES:CC(=O)Oc1ccc2c(c1)Oc1cc(ccc1C12c2cc(ccc2C(=O)O1)C(=O)O)OC(=O)C
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