CAS 33926-00-0
:o-Tolyl tetrazolium red 2,5-Diphenyl-3-o-tolyl-2H-tetrazolium chloride
Description:
o-Tolyl tetrazolium red, also known as 2,5-Diphenyl-3-o-tolyl-2H-tetrazolium chloride, is a chemical compound commonly used as a redox indicator and in various biological assays, particularly in cell viability and proliferation studies. This compound is characterized by its tetrazolium structure, which allows it to undergo reduction in living cells, leading to the formation of a colored formazan product. The presence of o-tolyl and diphenyl groups contributes to its solubility and stability in organic solvents. Typically, it appears as a red or reddish-brown powder, and its solubility is influenced by the pH of the solution. The compound is sensitive to light and should be stored in a cool, dark place to maintain its integrity. In laboratory settings, it is often utilized in assays such as the MTT assay, where it serves as a marker for metabolic activity. Safety precautions should be taken when handling this compound, as it may pose health risks if ingested or inhaled.
Formula:C20H17N4
InChI:InChI=1/C20H17N4/c1-16-10-8-9-15-19(16)24-22-20(17-11-4-2-5-12-17)21-23(24)18-13-6-3-7-14-18/h2-15H,1H3/q+1
SMILES:Cc1ccccc1n1nc(c2ccccc2)nn1c1ccccc1
Synonyms:- o-Tolyltetrazolium red
- 3-(2-methylphenyl)-2,5-diphenyl-2H-tetrazol-3-ium
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