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CAS 35340-49-9

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dihydrofluorescein diacetate 90-95%

Description:
Dihydrofluorescein diacetate (DHFDA) is a non-fluorescent compound that serves as a cell-permeable probe for detecting reactive oxygen species (ROS) within biological systems. Upon entering cells, DHFDA is hydrolyzed by intracellular esterases to produce dihydrofluorescein, which is highly fluorescent. This transformation allows for the visualization of oxidative stress in live cells. The substance is typically characterized by its high solubility in organic solvents and moderate solubility in water, making it suitable for various biological applications. DHFDA is often utilized in fluorescence microscopy and flow cytometry to assess cellular health and oxidative damage. Its purity, often reported between 90-95%, indicates the presence of minimal impurities, which is crucial for reproducibility in experimental results. Safety considerations include handling it with care, as with many chemical reagents, to avoid potential skin and eye irritation. Overall, DHFDA is a valuable tool in biochemistry and cell biology for studying oxidative stress and related cellular processes.
Formula:C24H18O7
InChI:InChI=1/C24H18O7/c1-13(25)29-15-7-9-19-21(11-15)31-22-12-16(30-14(2)26)8-10-20(22)23(19)17-5-3-4-6-18(17)24(27)28/h3-12,23H,1-2H3,(H,27,28)
SMILES:CC(=O)Oc1ccc2c(c1)Oc1cc(ccc1C2c1ccccc1C(=O)O)OC(=O)C
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