CAS 3625-57-8

Nile Blue A

Description:

Nile Blue A is a synthetic dye belonging to the class of phenothiazine dyes, characterized by its vibrant blue color. It is primarily used as a biological stain in microscopy and histology due to its ability to selectively bind to certain cellular components, particularly nucleic acids and lipids. The compound exhibits fluorescence, making it useful for imaging applications. Nile Blue A is soluble in organic solvents and has limited solubility in water, which influences its application in various biological systems. Its chemical structure includes a phenothiazine core, contributing to its stability and reactivity. The dye can undergo protonation, leading to changes in its spectral properties, which is significant for its use in pH-sensitive applications. Additionally, Nile Blue A has been studied for its potential applications in photodynamic therapy and as a marker in cellular studies. However, safety considerations should be taken into account, as with many synthetic dyes, due to potential toxicity and environmental impact.

Formula: C₄₀H₄₀N₆O₆S

InChI: InChI=1/C20H19N3O.H2O4S/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)/p+1

InChI key: InChIKey=MMYKGGIXVBGGSZ-UHFFFAOYSA-L

SMILES: S(=O)(=O)([O-])[O-].NC1=C2C(=C3C([O+]=C4C(=N3)C=CC(N(CC)CC)=C4)=C1)C=CC=C2

Synonyms:

• (9-Diethylaminobenzo[A]Phenoxazin-5-Ylidene)Ammonium
• 5-Amino-9-(diethylamino)benzo[a]phenoxazinium sulfate
• Benzo[a]phenoxazin-7-ium, 5-amino-9-(diethylamino)-, sulfate (2:1)
• Bis[5-amino-9-(diethylamino)benzo[a]phenazoxonium] sulfate
• Nile Blue A
• Nile Blue A sulfate
• Nile blue sulfate
• Sulfuric Acid
• bis[9-(diethylamino)-5H-benzo[a]phenoxazin-5-iminium] sulfate
• Nile Blue A,Basic Blue12, Nile blue sulfate
• Basic Blue 12, Nile Blue sulphate
• NILE BLUE, HYDROGEN SULFATE
• [a]phenoxazinium Sulfate
• Benzo[.alpha.]phenoxazin-7-ium,5-amino-9-(diethylamino)-,sulfate(2:1)
• BASIC BLUE 12 SULFATE SALT
• NILE BLUE A, FOR MICROSCOPY
• NILE BLUE A, CERTIFIED
• 5-amino-9-(diethylamino)-benzo(a)phenoxazin-7-iu sulfate
• NILE BLUE HYDROGEN SULPHATE
• Bis[5-amino-9-(diethylamino)benzo[a]
• NILE BLUE
• 9-(diethylamino)-5-imino-5h-benzo(a)phenazoxazinsulfate(2:1)
• 5-amino-9-(diethylamino)benzo
• NILE BLUE A SULFATE SALT
• Nile Blue A,pure,certified
• CI NO 51180
• bis[5-amino-9-(diethylamino)benzo[a]phenoxazin-7-ium] sulphate
• Basic Blue12, Nile blue sulfate
• BASIC BLUE 12
• Nile Blue A, Pure
• NileBlueSulphateForMicroscopy
• Benzo[.alpha.] phenoxazin-7-ium, 5-amino-9-(diethylamino)-, sulfate
• LABOTEST-BB LT00451959
• Resistance Ireland
• nileblueasulphate
• Nile Blue A, certified, pure
• CI 51180
• 5-Amino-9-(diethylamino)benzo[a]phenoxazine-7-ium·0.5sulfate
• Nile Blue A, certified, pure 10GR
• 5-amino-9-(diethylamino)-benzo(a)phenoxazin-7-iusulfate(2:1)
• phenazoxonium] Sulfate
• NileBlueC.I.51180
• Benzoaphenoxazin-7-ium, 5-amino-9-(diethylamino)-, sulfate (2:1)

Nile Blue A

CAS: 3625-57-8

Used in the preparation of an amperometric glucose sensor. Also can be employed in Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue; Lillie and Fuller method for fatty acids/lipids using frozen sections of fatty liver a

Formula: C₄₀H₄₀N₆O₆S

Color and Shape: Dark green to dark blue or purple or very dark brown, Powder

Molecular weight: 732.84

Ref: 02-A17174

5-Amino-9-(diethylamino)benzo[a]phenoxazinium sulfate

CAS: 3625-57-8

Formula: C₄₀H₄₀N₆O₆S

Purity: 75%

Color and Shape: Solid

Molecular weight: 732.8472

Ref: IN-DA00I76J

Nile Blue A

CAS: 3625-57-8

Nile Blue A

Formula: ·O₄S·2C₂₀H₂₀N₃O

Molecular weight: 732.85g/mol

Ref: 54-BIN0463

Nile Blue A (C.I. 51180)

CAS: 3625-57-8

Formula: C₄₀H₄₀N₆O₆S

Purity: ≥ 65.0%

Color and Shape: Green to black powder

Molecular weight: 732.84

Ref: 7W-GT3036

Nile Blue A sulfate

CAS: 3625-57-8

Nile Blue A differentiates melanins/lipofuscins, stains fats, and aids in glucose sensor prep.

Formula: C₂₀H₂₀N₃O₅S

Purity: 98%

Color and Shape: Dark Green To Black Powder

Molecular weight: 414.46

Ref: TM-T19034

Nile Blue Sulphate

CAS: 3625-57-8

Formula: C₄₀H₄₀N₆O₆S

Color and Shape: Dark bluish black, Powder

Molecular weight: 732.85

Ref: SR-62887

Nile blue A (Technical Grade)

Controlled Product

CAS: 3625-57-8

Applications Nile Blue A is used in the Lillie and Fullmer method for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue. Nile blue A is also useful for staining fats and phospholipids. Dyes and metabolites.
References Lillie, Stain Tech., 31,151 (1956). J.Histochem., 4,377 (1956). Z. Analyt. Chem.,260,290 (1972). For staining bone sections. Cytologie,11,261 (1940)

Formula: C₂₀H₂₀N₃O·O₄S

Color and Shape: Neat

Molecular weight: 732.85

Ref: TR-N464213