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CAS 369-07-3

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o-Nitrophenyl β-D-galactopyranoside

Description:
o-Nitrophenyl β-D-galactopyranoside (ONPG) is a synthetic compound commonly used as a substrate in biochemical assays, particularly for the detection of β-galactosidase activity. It is a glycoside, consisting of a galactopyranoside moiety linked to an o-nitrophenyl group. This compound is typically a white to pale yellow crystalline powder that is soluble in water and organic solvents. Upon hydrolysis by β-galactosidase, ONPG releases o-nitrophenol, which exhibits a distinct yellow color, allowing for easy spectrophotometric measurement of enzyme activity. The compound is stable under normal laboratory conditions but should be stored in a cool, dry place to prevent degradation. Its applications extend beyond enzyme assays, as it can also serve as a model substrate in studies of carbohydrate metabolism and enzyme kinetics. Safety precautions should be observed when handling ONPG, as it may pose health risks if ingested or inhaled.
Formula:C12H15NO8
InChI:InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
InChI key:InChIKey=KUWPCJHYPSUOFW-YBXAARCKSA-N
SMILES:O([C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)C2=C(N(=O)=O)C=CC=C2
Synonyms:
  • 2-Nitrophenyl Beta-D-Galactoside-(1,5)
  • 2-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactopyranoside
  • 2-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactoside
  • 2-Nitrophenyl β-D-galactopyranoside
  • 2-Nitrophenyl-Beta-D-Galactoside
  • 2-Nitrophenyl-Beta-D-galactopyranoside
  • 2-Nitrophenyl-alpha-D-galactoside
  • 2-Nitrophenylgalactoside
  • 2-nitrophenyl D-galactopyranoside
  • 2-nitrophenyl alpha-L-erythro-hexopyranoside
  • See more synonyms
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