CAS 369-07-3

o-Nitrophenyl β-D-galactopyranoside

Description:

o-Nitrophenyl β-D-galactopyranoside (ONPG) is a synthetic compound commonly used as a substrate in biochemical assays, particularly for the detection of β-galactosidase activity. It is a glycoside, consisting of a galactopyranoside moiety linked to an o-nitrophenyl group. This compound is typically a white to pale yellow crystalline powder that is soluble in water and organic solvents. Upon hydrolysis by β-galactosidase, ONPG releases o-nitrophenol, which exhibits a distinct yellow color, allowing for easy spectrophotometric measurement of enzyme activity. The compound is stable under normal laboratory conditions but should be stored in a cool, dry place to prevent degradation. Its applications extend beyond enzyme assays, as it can also serve as a model substrate in studies of carbohydrate metabolism and enzyme kinetics. Safety precautions should be observed when handling ONPG, as it may pose health risks if ingested or inhaled.

Formula: C₁₂H₁₅NO₈

InChI: InChI=1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1

InChI key: InChIKey=KUWPCJHYPSUOFW-YBXAARCKSA-N

SMILES: O([C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)C2=C(N(=O)=O)C=CC=C2

Synonyms:

• 2-Nitrophenyl Beta-D-Galactoside-(1,5)
• 2-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactopyranoside
• 2-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactoside
• 2-Nitrophenyl β-D-galactopyranoside
• 2-Nitrophenyl-Beta-D-Galactoside
• 2-Nitrophenyl-Beta-D-galactopyranoside
• 2-Nitrophenyl-alpha-D-galactoside
• 2-Nitrophenylgalactoside
• 2-nitrophenyl D-galactopyranoside
• 2-nitrophenyl alpha-L-erythro-hexopyranoside
• 2-nitrophenyl beta-L-glucopyranoside
• 2-nitrophenyl β-D-galactoside
• Galactopyranoside, 2-nitrophenyl
• Galactopyranoside, o-nitrophenyl, β-<span class="text-smallcaps">D</span>-
• NSC 83631
• Onpg
• o-NPG
• o-Nitrophenol β-<span class="text-smallcaps">D</span>-galactopyranoside
• o-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactopyranoside
• o-Nitrophenyl β-<span class="text-smallcaps">D</span>-galactoside
• o-Nitrophenyl β-galactoside
• o-Nitrophenyl-β-galactopyranoside
• ortho-Nitrophenyl-beta-D-galactoside
• β-<span class="text-smallcaps">D</span>-Galactopyranoside, 2-nitrophenyl
• o-Nitrophenyl β-D-galactopyranoside
• β-D-Galactopyranoside, 2-nitrophenyl

2-Nitrophenyl β-D-Galactopyranoside [Substrate for β-D-Galactosidase]

CAS: 369-07-3

Formula: C₁₂H₁₅NO₈

Purity: >98.0%(HPLC)

Color and Shape: White to Light yellow powder to crystaline

Molecular weight: 301.25

Ref: 3B-N0418

2-Nitrophenyl-β-D-galactopyranoside, 98+%

CAS: 369-07-3

2-Nitrophenyl-beta-D-galactopyranoside is a -Galactosidase substrate for colorimetric and EIA applications; counterpart of widely employed pNPP/alkaline phosphatase substrate. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation

Formula: C₁₂H₁₅NO₈

Purity: 98+%

Color and Shape: White to pale cream to pale yellow, Powder

Molecular weight: 301.25

Ref: 02-L10939

2-Nitrophenyl β-D-Galactopyranoside

CAS: 369-07-3

Formula: C₁₂H₁₅NO₈

Purity: 97%

Color and Shape: Solid

Molecular weight: 301.2494

Ref: IN-DA00I7AV

2-Nitrophenyl-β-D-galactopyranoside

CAS: 369-07-3

2-Nitrophenyl-β-D-galactopyranoside

Formula: C₁₂H₁₅NO₈

Purity: >99%

Color and Shape: off-white powder

Molecular weight: 301.2494g/mol

Ref: 54-BIM1202

ONPG

CAS: 369-07-3

ONPG

Purity: ≥98%

Molecular weight: 301.25g/mol

Ref: 54-BUP19632

2-Nitrophenyl b-D-galactopyranoside

CAS: 369-07-3

Formula: C₁₂H₁₅NO₈

Purity: ≥ 98.0%

Color and Shape: White to off-white powder

Molecular weight: 301.25

Ref: 7W-GC0605

2-Nitrophenyl β-D-Galactoside

CAS: 369-07-3

Formula: C₁₂H₁₅NO₈

Molecular weight: 301.08

Ref: 4Z-G-163017

ONPG

CAS: 369-07-3

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric and spectrophotometric substrate used to detect β-galactosidase activity.

Formula: C₁₂H₁₅NO₈

Purity: 99.82% - 99.91%

Color and Shape: White Crystalline Solid

Molecular weight: 301.25

Ref: TM-T19497

o-Nitrophenyl b-D-Galactopyranoside

Controlled Product

CAS: 369-07-3

Applications o-Nitrophenyl β-D-Galactopyranoside (cas# 369-07-3) is a compound useful in organic synthesis.

Formula: C₁₂H₁₅NO₈

Color and Shape: Neat

Molecular weight: 301.25

Ref: TR-N503580

2-Nitrophenyl-β-D-galactopyranoside - non-animal origin

CAS: 369-07-3

2-Nitrophenyl-beta-D-galactopyranoside is a non-animal origin chromogenic substrate for the detection of beta-galactosidase activity. It is used in food testing, diagnostics, and environmental testing. 2-Nitrophenyl-beta-D-galactopyranoside is also used as a ligand in enzyme assays and a fluorogenic substrate for chemiluminescent reactions. This product is conjugated to HRP.

Formula: C₁₂H₁₅NO₈

Purity: Min. 98 Area-%

Molecular weight: 301.25 g/mol

Ref: 3D-N-4195

2-Nitrophenyl-β-D-galactopyranoside

CAS: 369-07-3

Chromogenic β-galactosidase substrate yielding a yellow solution upon cleavage. Can be used for the detection of coliforms due to their beta-galactosidase activity.

Formula: C₁₂H₁₅NO₈

Purity: Min. 99.0 Area-%

Molecular weight: 301.26 g/mol

Ref: 3D-N-4190

o-Nitrophenyl-β-D-galactopyranoside

CAS: 369-07-3

M02731 - o-Nitrophenyl-beta-D-galactopyranoside

Formula: C₁₂H₁₅NO₈

Purity: 97%

Color and Shape: Solid

Molecular weight: 301.251

Ref: 10-M02731

o-Nitrophenyl-ß-D-Galacto Pyranoside (ONPG) extrapure, 99%

CAS: 369-07-3

Formula: C₁₂H₁₅NO₈

Purity: min. 99%

Color and Shape: White to pale yellow, Crystalline powder, Clear, Colourless to pale yellow

Molecular weight: 301.25

Ref: SR-66398