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CAS 39450-01-6

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Proteinase K

Description:
Proteinase K is a serine protease enzyme widely used in molecular biology for its ability to digest proteins and facilitate the extraction of nucleic acids. It is derived from the fungus Tritirachium album and is characterized by its broad substrate specificity, allowing it to cleave peptide bonds adjacent to both aliphatic and aromatic amino acids. Proteinase K is particularly effective at functioning in a wide range of pH levels and temperatures, making it a versatile tool in various laboratory applications. It is commonly employed in DNA and RNA purification protocols, as it can effectively degrade proteins that may interfere with nucleic acid extraction. Additionally, Proteinase K is resistant to inhibition by detergents and can remain active in the presence of chaotropic agents, which further enhances its utility in nucleic acid isolation. The enzyme is typically supplied as a lyophilized powder and is reconstituted in buffer solutions for use in experiments. Its stability and efficiency make it a staple in molecular biology research and diagnostics.
Formula:Unspecified
Synonyms:
  • E.C. 3.4.21.64
  • Endopeptidase K
  • Prok
  • Protease K
  • Proteinase K
  • Proteinase, Tritirachium album-Serin→
  • Tritirachium album proteinase K
  • proteinasa, serina de Tritirachium album
  • proteinase, serine, Tritirachium album
  • proteinase K crude
  • See more synonyms
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