CAS 4178-93-2

Leucine p-nitroanilide

Description:

Leucine p-nitroanilide, with the CAS number 4178-93-2, is a synthetic compound commonly used in biochemical applications, particularly in the study of proteolytic enzymes. It is an amino acid derivative, specifically a peptide bond formed between leucine and p-nitroaniline. This compound is characterized by its ability to act as a substrate for various enzymes, allowing researchers to investigate enzyme activity and kinetics. Leucine p-nitroanilide is typically a white to off-white crystalline solid, and it is soluble in organic solvents, which facilitates its use in laboratory settings. The presence of the p-nitroanilide group provides a chromogenic property, enabling the detection of enzymatic activity through colorimetric assays. This compound is also notable for its stability under standard laboratory conditions, making it a reliable choice for experimental procedures. As with many chemical substances, proper handling and safety precautions should be observed, as it may pose health risks if ingested or improperly managed.

Formula: C₁₂H₁₇N₃O₃

InChI: InChI=1S/C12H17N3O3/c1-8(2)7-11(13)12(16)14-9-3-5-10(6-4-9)15(17)18/h3-6,8,11H,7,13H2,1-2H3,(H,14,16)/t11-/m0/s1

InChI key: InChIKey=AXZJHDNQDSVIDR-NSHDSACASA-N

SMILES: N(C([C@H](CC(C)C)N)=O)C1=CC=C(N(=O)=O)C=C1

Synonyms:

• (2S)-2-Amino-4-methyl-N-(4-nitrophenyl)pentanamide
• (2S)-4-methyl-1-[(4-nitrophenyl)amino]-1-oxopentan-2-aminium
• <span class="text-smallcaps">L</span>-Leucine p-nitroanilide
• <span class="text-smallcaps">L</span>-Leucyl-4-nitroanilide
• <span class="text-smallcaps">L</span>-Leucyl-p-nitroanilide
• H-Leu-Pna
• L-Leucine-p-nitroanilide
• Leucine p-nitroanilide
• N-(4-nitrophenyl)-L-leucinamide
• N-<span class="text-smallcaps">L</span>-Leucyl-p-nitroanilide
• N-α-<span class="text-smallcaps">L</span>-Leucine p-nitroanilide
• Pentanamide, 2-amino-4-methyl-N-(4-nitrophenyl)-, (2S)-
• Pentanamide, 2-amino-4-methyl-N-(4-nitrophenyl)-, (S)-
• Valeranilide, 2-amino-4-methyl-4′-nitro-, <span class="text-smallcaps">L</span>-
• Valeranilide, 2-amino-4-methyl-4′-nitro-, L-

H-Leu-pNA

CAS: 4178-93-2

Substrate for aminopeptidase M and leucine aminopeptidase.

Formula: C₁₂H₁₇N₃O₃

Purity: > 99%

Color and Shape: Light Yellow

Molecular weight: 251.29

Ref: 01-4001072

H-LEU-PNA

CAS: 4178-93-2

Formula: C₁₂H₁₇N₃O₃

Purity: 98%

Color and Shape: Solid

Molecular weight: 251.2817

Ref: IN-DA00BY9Y

L-Leucyl-4-nitroanilide

CAS: 4178-93-2

L-Leucyl-4-nitroanilide

Purity: 98%

Ref: 54-OR1037775

L-Leucine-p-nitroanilide

CAS: 4178-93-2

L-Leucine-p-nitroanilide is a substrate. It is used for the colorimetric determination of leucine aminopeptidase.

Formula: C₁₂H₁₇N₃O₃

Color and Shape: Crystalline Colourless Liquid

Molecular weight: 251.28

Ref: TM-T20445

L-Leucine 4-nitroanilide

CAS: 4178-93-2

L-Leucine 4-nitroanilide serves as a substrate for the colorimetric determination of leucine aminopeptidase. The application of L-Leucine-p-nitroanilide includes its utilization as a substrate in studies assessing the intestinal leucine aminopeptidase activity of juvenile rainbow trout (Oncorhynchus mykiss). Additionally, it plays a role in insulin-regulated aminopeptidase inhibition assays and is employed to determine exopeptidase activity.

Formula: C₁₂H₁₇N₃O₃

Purity: (Titration) 99 To 101%

Color and Shape: Powder

Molecular weight: 251.28 g/mol

Ref: 3D-FL47691

(S)-2-Amino-4-methyl-N-(4-nitrophenyl)pentanamide

CAS: 4178-93-2

Formula: C₁₂H₁₇N₃O₃

Purity: 98%

Color and Shape: Liquid, No data available.

Molecular weight: 251.286

Ref: 10-F463589

Leu-pNA

CAS: 4178-93-2

Leu-pNA is a protein synthesis inhibitor that binds to the active site of the enzyme peptidyl prolyl cis-trans isomerase (PPIase). This inhibitor prevents the enzyme from catalyzing the conversion of proline residues in peptides to their cis or trans isomers. Leu-pNA has been shown to inhibit proteolytic enzymes such as soybean trypsin and activated proteases, and also has an inhibitory effect on polymerase chain reaction (PCR) enzyme activities. The binding of Leu-pNA to PPIase can be reversed by heating at 60°C for 20 minutes.

Formula: C₁₂H₁₇N₃O₃

Purity: Min. 95%

Molecular weight: 251.28 g/mol

Ref: 3D-SLN-3014

L-Leucine-4-nitroanilide, 99%

CAS: 4178-93-2

It is used to detect/evaluate functionality of amino peptidases. The compound is used in an ELISA assay to detect biological functionality of chicken amino peptidase produced by E. coli. It is also employed to characterize the native IRAP (placental leucine amino peptidase). Leu-pNA, Chromogenic Sub

Formula: C₁₂H₁₇N₃O₃

Purity: 99%

Color and Shape: Crystals or powder or crystalline powder, White to cream to pale yellow

Molecular weight: 251.29

Ref: 02-A11994