CAS 494-38-2

Acridine Orange base

Description:

Acridine Orange base, with the CAS number 494-38-2, is a synthetic organic compound belonging to the acridine family. It is primarily used as a fluorescent dye and is known for its ability to intercalate into nucleic acids, making it valuable in biological and medical research, particularly in the staining of cells and tissues for microscopy. The compound exhibits a strong fluorescence under ultraviolet light, allowing for the visualization of cellular components. Acridine Orange can exist in both a protonated and unprotonated form, depending on the pH of the environment, which influences its spectral properties. It is soluble in water and organic solvents, making it versatile for various applications. Additionally, Acridine Orange is utilized in the differentiation of live and dead cells, as it can penetrate live cells and emit green fluorescence, while dead cells exhibit red fluorescence due to the binding of the dye to DNA. Safety precautions should be taken when handling this compound, as it may pose health risks upon exposure.

Formula: C₁₇H₁₉N₃

InChI: InChI=1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3

InChI key: InChIKey=DPKHZNPWBDQZCN-UHFFFAOYSA-N

SMILES: N(C)(C)C1=CC2=C(C=C3C(=N2)C=C(N(C)C)C=C3)C=C1

Synonyms:

• 3,6-Acridinediamine, N,N,N′,N′-tetramethyl-
• 3,6-Acridinediamine, N³,N³,N⁶,N⁶-tetramethyl-
• 3,6-Bis(dimethylamino)acridine
• 3,6-Di(dimethylamino)acridine
• 3-N,3-N,6-N,6-N-Tetramethylacridine-3,6-diamine
• Acridine orange free base
• Acridine, 3,6-bis(dimethylamino)-
• Brilliant Acridine Orange E
• N,N,N',N'-tetramethylacridine-3,6-diamine
• N,N,N,N-tetramethyl(acridine-3,6-diyldiamine
• N³,N³,N⁶,N⁶-Tetramethyl-3,6-acridinediamine
• NSC 194350
• Solvent Orange 15
• Waxoline Orange A
• C.I. No. 46005:1
• 3,6-Acridinediamine, N3,N3,N6,N6-tetramethyl-
• C.I. Solvent Orange 15
• C.I. 46005B

ACRIDINE ORANGE

CAS: 494-38-2

Formula: C₁₇H₁₉N₃

Purity: 75%

Color and Shape: Solid

Molecular weight: 265.3529

Ref: IN-DA00DEEC

Acridine Orange base

CAS: 494-38-2

Acridine Orange base is a cell-permeable fluorescent dye which exhibits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA/ssRNA .

Formula: C₁₇H₁₉N₃

Purity: 93.89%

Color and Shape: Solid

Molecular weight: 265.35

Ref: TM-T75338

Acridine Orange base

CAS: 494-38-2

Formula: C₁₇H₁₉N₃

Color and Shape: Orange to brown powder

Molecular weight: 265.35

Ref: 7W-GT9731

N3,N3,N6,N6-Tetramethylacridine-3,6-Diamine

CAS: 494-38-2

N3,N3,N6,N6-Tetramethylacridine-3,6-Diamine

Purity: 90%

Molecular weight: 265.35g/mol

Ref: 54-OR1014212

Acridine orange base

CAS: 494-38-2

Acridine orange is a fluorescent dye that can pass through the cell membrane and bind to the cytoplasmic structures of cells. It can be used as a probe for determining the presence of cell nuclei, mitochondria, and other organelles. Acridine orange is also used in experiments to determine mitochondrial membrane potential. In this type of experiment, acridine orange is added to cells and its uptake into mitochondria is observed. The dye changes color from orange to green when it binds with the mitochondria because it emits green fluorescence at higher excitation wavelengths than red fluorescence. Acridine orange has been shown to increase fatty acid synthesis by binding with fatty acid synthase, which is an enzyme involved in fatty acid biosynthesis. Acridine orange also increases ATP levels by inhibiting oxidative phosphorylation in mitochondria.

Formula: C₁₇H₁₉N₃

Color and Shape: Orange Powder

Molecular weight: 265.35 g/mol

Ref: 3D-FA39736

N3,N3,N6,N6-Tetramethylacridine-3,6-diamine

CAS: 494-38-2

Formula: C₁₇H₁₉N₃

Purity: Dye content 75

Color and Shape: Solid

Molecular weight: 265.36

Ref: 10-F240163

Acridine Orange Stain

Controlled Product

CAS: 494-38-2

Formula: C₁₇H₁₉N₃

Color and Shape: Orange

Molecular weight: 265.36

Ref: TR-A190933