CAS 53046-98-3

N-cbz-gly-gly-leu P-nitroanilide

Description:

N-Cbz-gly-gly-leu P-nitroanilide is a synthetic compound commonly used in biochemical research, particularly in the study of proteolytic enzymes. This compound features a Cbz (carbobenzyloxy) protecting group, which enhances its stability and solubility, making it suitable for various applications in peptide synthesis and enzyme assays. The structure includes two glycine residues and a leucine residue, contributing to its peptide characteristics. The P-nitroanilide moiety serves as a chromogenic substrate, allowing for the detection of enzymatic activity through colorimetric changes. This compound is typically utilized in assays to measure the activity of serine proteases and other enzymes that cleave peptide bonds. Its characteristics include moderate solubility in organic solvents and water, and it is generally stable under standard laboratory conditions. However, like many chemical substances, it should be handled with care, following appropriate safety protocols to mitigate any potential hazards associated with its use.

Formula: C₂₄H₂₉N₅O₇

InChI: InChI=1/C24H29N5O7/c1-16(2)12-20(27-18-8-10-19(11-9-18)29(34)35)23(32)28-22(31)14-25-21(30)13-26-24(33)36-15-17-6-4-3-5-7-17/h3-11,16,20,27H,12-15H2,1-2H3,(H,25,30)(H,26,33)(H,28,31,32)/t20-/m0/s1

SMILES: CC(C)C[C@@H](C(=O)N=C(CN=C(CN=C(O)OCc1ccccc1)O)O)Nc1ccc(cc1)N(=O)=O

Synonyms:

• Z-Gly-Gly-Leu-pNA
• N-[(benzyloxy)carbonyl]glycyl-N-{(2S)-4-methyl-2-[(4-nitrophenyl)amino]pentanoyl}glycinamide

Z-Gly-Gly-Leu-pNA

CAS: 53046-98-3

The chromogenic substrate Z-GGL-pNA is used alone or in combination with other substrates as Suc-LLVY-AMC for measuring the chymotrypsin-like activity of the proteasome. Z-GGL-pNA is also cleaved by proteinase yscE (kexin), subtilisins, and neutral endopeptidase 24.5.

Formula: C₂₄H₂₉N₅O₇

Purity: > 99%

Color and Shape: White

Molecular weight: 499.52

Ref: 01-4002941

Z-Gly-Gly-Leu-pNA

CAS: 53046-98-3

Z-Gly-Gly-Leu-pNA is a peptide that is derived from the amino acid sequence of a basic protein. It can be used as a fluorescent probe to detect hydration and enhance the fluorescence of other proteins. Z-Gly-Gly-Leu-pNA has been shown to bind basic protein subunits with high affinity, forming a stable complex. This peptide has also been shown to hydrolyze in acidic pH, making it useful for detecting proteolytic activity. The optimum pH for this peptide is neutral, at around 7.5. This peptide can be solubilized by adding Triton X or Brij 35 detergent and stored at -20°C.

Formula: C₂₄H₂₉N₅O₇

Purity: Min. 95%

Molecular weight: 499.52 g/mol

Ref: 3D-SGL-3111

Z-Gly-Gly-Leu-pNA

CAS: 53046-98-3

The peptide z-Gly-Gly-Leu-pNA is a synthetic substrate that is used in the study of metalloendopeptidases. The peptide is composed of four amino acids and has an acidic, monoclonal antibody, dodecyl, proteolytic, peptide hormones, extracellular, inactivated, serine protease. It is synthesized from wheat leaves and can be used as a substrate for the enzyme.

Formula: C₂₄H₂₉N₅O₇

Purity: Min. 95%

Molecular weight: 499.52 g/mol

Ref: 3D-FG110718